Nogi Y, Kato C, Horikoshi K
Deep Star Group, Japan Marine Science and Technology Center, Yokosuka, Japan.
Arch Microbiol. 1998 Oct;170(5):331-8. doi: 10.1007/s002030050650.
Several barophilic Shewanella species have been isolated from deep-sea sediments at depths of 2,485-6,499 m. From the results of taxonomic studies, all of these isolates have been identified as strains of Shewanella benthica except for strain DSS12. Strain DSS12 is a member of a novel, moderately barophilic Shewanella species isolated from the Ryukyu Trench at a depth of 5,110 m. On Marine Agar 2216 plates, this organism produced a violet pigment, whereas the colonies of other isolates (S. benthica) were rose-colored. Phylogenetic analysis based on 16 S ribosomal RNA gene sequences showed that strain DSS12 represents a separate lineage within the genus Shewanella that is closely related to S. benthica and particularly to the members of the Shewanella barophiles branch. The temperature range for growth and some of the biochemical characteristics indicate that strain DSS12 differs from other Shewanella species. Furthermore, strain DSS12 displayed a low level of DNA similarity to the Shewanella type strains. Based on these differences, it is proposed that strain DSS12 represents a new deep-sea Shewanella species. The name Shewanella violacea (JCM 10179) is proposed.
几种嗜压希瓦氏菌已从深度为2485 - 6499米的深海沉积物中分离出来。从分类学研究结果来看，除了菌株DSS12外，所有这些分离株都被鉴定为底栖希瓦氏菌菌株。菌株DSS12是从琉球海沟5110米深处分离出的一种新型中度嗜压希瓦氏菌的成员。在海生琼脂2216平板上，这种微生物产生了一种紫色色素，而其他分离株（底栖希瓦氏菌）的菌落是玫瑰色的。基于16S核糖体RNA基因序列的系统发育分析表明，菌株DSS12代表希瓦氏菌属内一个独立的谱系，与底栖希瓦氏菌密切相关，尤其与嗜压希瓦氏菌分支的成员相关。生长的温度范围和一些生化特性表明菌株DSS12与其他希瓦氏菌物种不同。此外，菌株DSS12与希瓦氏菌模式菌株的DNA相似性水平较低。基于这些差异，有人提出菌株DSS12代表一种新的深海希瓦氏菌物种。建议将其命名为紫色希瓦氏菌（JCM 10179）。
