Kato C, Li L, Nogi Y, Nakamura Y, Tamaoka J, Horikoshi K
Deep Star Group, Japan Marine Science and Technology Center, Yokosuka, Japan.
Appl Environ Microbiol. 1998 Apr;64(4):1510-3. doi: 10.1128/AEM.64.4.1510-1513.1998.
Two strains of obligately barophilic bacteria were isolated from a sample of the world's deepest sediment, which was obtained by the unmanned deep-sea submersible Kaiko in the Mariana Trench, Challenger Deep, at a depth of 10,898 m. From the results of phylogenetic analysis based on 16S rRNA gene sequences, DNA-DNA relatedness study, and analysis of fatty acid composition, the first strain (DB21MT-2) appears to be most highly similar to Shewanella benthica and close relatives, and the second strain (DB21MT-5) appears to be closely related to the genus Moritella. The optimal pressure conditions for growth of these isolates were 70 MPa for strain DB21MT-2 and 80 MPa for strain DB21MT-5, and no growth was detected at pressures of less than 50 MPa with either strain. This is the first evidence of the existence of an extreme-barophile bacterium of the genus Moritella isolated from the deep-sea environment.
从世界最深沉积物样本中分离出两株专性嗜压细菌，该样本由无人深海潜水器“海沟号”在马里亚纳海沟挑战者深渊10898米深处获取。基于16S rRNA基因序列的系统发育分析、DNA-DNA相关性研究以及脂肪酸组成分析结果表明，第一株菌（DB21MT-2）似乎与底栖希瓦氏菌及其近缘菌最为相似，第二株菌（DB21MT-5）似乎与莫氏菌属密切相关。这些分离菌株生长的最佳压力条件是，DB21MT-2菌株为70兆帕，DB21MT-5菌株为80兆帕，两种菌株在压力低于50兆帕时均未检测到生长。这是首次从深海环境中分离出莫氏菌属极端嗜压细菌的证据。
