Palanichelvam K, Kunik T, Citovsky V, Gafni Y
Department of Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
J Gen Virol. 1998 Nov;79 ( Pt 11):2829-33. doi: 10.1099/0022-1317-79-11-2829.
The capsid protein (CP) of tomato yellow leaf curl virus (TYLCV) is the only known component of the virus coat. Here, we identify TYLCV CP as a single-stranded (ss) DNA binding protein. Purified TYLCV CP bound ssDNA in a highly cooperative and sequence-nonspecific fashion. TYLCV CP-ssDNA complexes were resistant to nucleolytic digestion and remained stable at relatively high salt concentrations. Because TYLCV CP is known to contain an active nuclear targeting signal, we propose that its association with the viral genomic ssDNA mediates TYLCV entry into the host cell nucleus during the infection process.
番茄黄化曲叶病毒（TYLCV）的衣壳蛋白（CP）是已知的病毒外壳唯一组成成分。在此，我们鉴定出TYLCV CP为一种单链（ss）DNA结合蛋白。纯化的TYLCV CP以高度协同且序列非特异性的方式结合ssDNA。TYLCV CP-ssDNA复合物对核酸酶消化具有抗性，并且在相对高盐浓度下保持稳定。由于已知TYLCV CP含有一个活性核定位信号，我们提出其与病毒基因组ssDNA的结合在感染过程中介导TYLCV进入宿主细胞核。
