Chan B P, Fu S C, Qin L, Rolf C, Chan K M
Department of Orthopaedics and Traumatology, Chinese University of Hong Kong, Shatin, NT.
J Orthop Res. 1998 Sep;16(5):597-603. doi: 10.1002/jor.1100160512.
The ultimate stress of the central one-third of the patellar tendon was studied in a gap wound-healing model in the rat. The specimens were also analyzed for collagen and nonreducible crosslinks, as measured by hydroxyproline and pyridinoline content, respectively. Thirty days after injury, the ultimate stress of the healing patellar tendon was restored to an average of 71% of the control value and remained constant over time. The pyridinoline content of the healing tendon was twice the control value by 30 days after injury and reached a plateau; however, the hydroxyproline content did not change significantly over time. Stepwise regression analysis demonstrated that pyridinoline was a better biochemical marker for ultimate stress than was hydroxyproline. The current study provides insights into the functional behaviour of the healing patellar tendon by establishing the relationship between the two biochemical components and the ultimate stress of the healing patellar tendon. This study also suggests the possibility of using pyridinoline content as an indirect marker of the ultimate stress because in vivo assessment is impossible.
在大鼠的间隙愈合模型中，研究了髌腱中央三分之一的极限应力。还分别通过羟脯氨酸和吡啶啉含量对标本进行了胶原蛋白和不可还原交联的分析。损伤后30天，愈合中的髌腱极限应力恢复至对照值的平均71%，并随时间保持恒定。损伤后30天，愈合肌腱的吡啶啉含量是对照值的两倍，并达到稳定水平；然而，羟脯氨酸含量随时间没有显著变化。逐步回归分析表明，吡啶啉比羟脯氨酸更适合作为极限应力的生化标志物。本研究通过建立两种生化成分与愈合髌腱极限应力之间的关系，深入了解了愈合髌腱的功能行为。该研究还表明，由于无法进行体内评估，使用吡啶啉含量作为极限应力的间接标志物是有可能的。
