Deplazes P, Grimm F, Papaprodromou M, Cavaliero T, Gramiccia M, Christofi G, Christofi N, Economides P, Eckert J
Institute of Parasitology, University of Zurich, Switzerland.
Acta Trop. 1998 Sep 15;71(2):169-78. doi: 10.1016/s0001-706x(98)00064-3.
During a serological survey in 1996, a total of 601 dogs (group I) distributed all over the government controlled southern area of Cyprus was examined using an enzyme-linked immunosorbent assay (ELISA) for the presence of specific antibodies directed against soluble antigens of promastigote stages of Leishmania infantum. The overall seroprevalence rate in this group was 1.7%. A second group (group II) of dogs was selected from regions where seropositive dogs where determined within the first group. In group II specific anti-Leishmania antibodies could be detected in 30 of 301 dogs investigated (10%). The highest seroprevalence rates were found in the regions of Agios Georgios (26.2%) and Limnatis (12.2%). Ten parasite isolates from ten dogs (six with typical symptoms of canine leishmaniosis and four without symptoms) originating from five locations could be characterised by zymodeme analysis. All ten isolates were identified as L. infantum zymodeme MON 1.
在1996年的一项血清学调查中，使用酶联免疫吸附测定（ELISA）对塞浦路斯政府控制的南部地区各地的601只狗（第一组）进行了检查，以检测针对婴儿利什曼原虫前鞭毛体阶段可溶性抗原的特异性抗体。该组的总体血清阳性率为1.7%。第二组狗（第二组）从第一组中确定血清阳性狗的地区选取。在第二组中，在所调查的301只狗中有30只检测到特异性抗利什曼原虫抗体（10%）。血清阳性率最高的地区是阿吉奥斯·乔治奥斯（26.2%）和利姆纳蒂斯（12.2%）。对来自五个地点的十只狗（六只患有犬利什曼病典型症状，四只无症状）的十个寄生虫分离株进行了酶谱分析。所有十个分离株均被鉴定为婴儿利什曼原虫酶谱MON 1。
