对肠杆菌科耐庆大霉素菌株中庆大霉素、卡那霉素、妥布霉素和阿米卡星耐药机制的分析。
Analysis of neomycin, kanamycin, tobramycin and amikacin resistance mechanisms in gentamicin-resistant isolates of Enterobacteriaceae.
作者信息
Adwan K, Abu-Hasan N, Al-Asmar H
机构信息
Department of Biological Sciences, An-Najah National University, Nablus, Palestine.
出版信息
J Med Microbiol. 1998 Nov;47(11):1019-21. doi: 10.1099/00222615-47-11-1019.
Twenty-four gentamicin-resistant isolates of Enterobacteriaceae, obtained from the clinical laboratories of three health centres in Nablus, Palestine, were tested for susceptibility to neomycin, kanamycin, tobramycin and amikacin. Resistance rates were 29.2% for neomycin, 58.3% for kanamycin, 45.8% for tobramycin and 8.3% for amikacin. Fourteen (58.3%) isolates were noted to be multiresistant, i.e., resistant to gentamicin and two or more other aminoglycosides; resistance to gentamicin, kanamycin and tobramycin was the most common pattern of multiple resistance. This pattern implies the involvement of adenyltransferase ANT(")-I activity. Plasmid profiles and curing experiments suggested a plasmid localisation of gentamicin, neomycin, kanamycin and tobramycin resistance genes. However, a chromosomal location is proposed for plasmid-deficient strains. Cross-resistance in two isolates to all aminoglycosides tested suggested membrane impermeability to aminoglycosides as the mechanism of resistance.
从巴勒斯坦纳布卢斯的三个健康中心的临床实验室获得了24株对庆大霉素耐药的肠杆菌科细菌，对其进行了新霉素、卡那霉素、妥布霉素和阿米卡星的敏感性测试。新霉素的耐药率为29.2%，卡那霉素为58.3%，妥布霉素为45.8%，阿米卡星为8.3%。有14株（58.3%）分离株被发现具有多重耐药性，即对庆大霉素和两种或更多其他氨基糖苷类耐药；对庆大霉素、卡那霉素和妥布霉素的耐药是最常见的多重耐药模式。这种模式表明腺苷转移酶ANT(“) - I活性的参与。质粒图谱和消除实验表明庆大霉素、新霉素、卡那霉素和妥布霉素耐药基因定位于质粒。然而，对于缺乏质粒的菌株，提出其耐药基因定位于染色体。两株分离株对所有测试氨基糖苷类均有交叉耐药，提示氨基糖苷类药物的膜通透性障碍是其耐药机制。
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