Mukasa A, Yoshida H, Kobayashi N, Matsuzaki G, Nomoto K
Center for Neurologic Diseases, Brigham and Women's Hospital, MA, Boston, USA; Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Immunology. 1998 Nov;95(3):395-401. doi: 10.1046/j.1365-2567.1998.00585.x.
In a previous report, we investigated inflammatory responses induced by injecting Listeria monocytogenes into one testis of a mouse. We demonstrated that the contralateral testis also developed an orchitis despite the absence of bacteria, indicating that the inflammation on the uninfected, contralateral side was of autoimmune character. In both infected and autoimmune testes, gammadelta and alphabeta T cells infiltrated during the inflammation. In this paper, we present the data of a comparison of the character of gammadelta T cells of the infected and autoimmune testes. In both testes, gammadelta T cells appeared to be activated, as assessed by high CD44 and low l-selectin expression. Analysis of T-cell receptor (TCR) usage in both inflammation types revealed the same gammadelta TCR repertoire. Finally, the semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that gammadelta T cells in both types of inflammation were capable of producing interleukin-2 (IL-2), IL-4, interferon-gamma (IFN-gamma), IL-10 and transforming growth factor-beta (TGF-beta). These results imply that gammadelta T cells present in infected-induced and autoimmunity-induced inflammation have the same characteristics and could work as immunoregulatory cells.
在之前的一份报告中，我们研究了将单核细胞增生李斯特菌注射到小鼠一侧睾丸所诱导的炎症反应。我们证明，尽管对侧睾丸没有细菌，但也会发生睾丸炎，这表明未感染的对侧睾丸的炎症具有自身免疫特征。在感染性和自身免疫性睾丸炎中，γδ和αβ T细胞在炎症过程中浸润。在本文中，我们展示了感染性和自身免疫性睾丸炎中γδ T细胞特征比较的数据。在两个睾丸中，通过高CD44和低L-选择素表达评估，γδ T细胞似乎都被激活。对两种炎症类型中T细胞受体（TCR）使用情况的分析揭示了相同的γδ TCR库。最后，半定量逆转录聚合酶链反应（RT-PCR）表明，两种炎症类型中的γδ T细胞都能够产生白细胞介素-2（IL-2）、IL-4、干扰素-γ（IFN-γ）、IL-10和转化生长因子-β（TGF-β）。这些结果意味着，感染诱导和自身免疫诱导炎症中存在的γδ T细胞具有相同的特征，并且可以作为免疫调节细胞发挥作用。
