Benveniste O, Martin M, Villinger F, Dormont D
Service de Neurovirologie (DSV/DRM), CRSSA, CEA, Institut Paris-Sud sur les Cytokines, Fontenay aux Roses, France.
Cytokines Cell Mol Ther. 1998 Sep;4(3):207-14.
Various methods are currently used to monitor cytokine mRNA expression levels in clinical samples. In instances where sample size is limited (e.g. biopsies), amplification procedures such as reverse transcription followed by polymerase chain reaction amplification appear to be the only ones that are currently useful. This paper provides a review of such techniques for the detection and quantitation of cytokine message in clinical samples, and compares semiquantitative and competitive techniques in terms of sensitivity, reproducibility, feasibility and costs.
目前有多种方法用于监测临床样本中细胞因子mRNA的表达水平。在样本量有限的情况下（如活检），诸如逆转录后进行聚合酶链反应扩增的扩增程序似乎是目前唯一有用的方法。本文综述了检测和定量临床样本中细胞因子信息的此类技术，并在灵敏度、可重复性、可行性和成本方面对半定量技术和竞争性技术进行了比较。
