Li N, Anderson W K, Bhawan J
Department of Dermatology, Boston University School of Medicine, Massachusetts, USA.
J Cutan Pathol. 1998 Sep;25(8):413-9. doi: 10.1111/j.1600-0560.1998.tb01767.x.
Recently, a new herpesvirus-like DNA sequence named Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV8) has been isolated from almost all cases of Kaposi's sarcoma (KS). It has not been found in most benign and malignant cutaneous hemangioproliferative disorders other than KS. To further verify the specificity of the association of this new viral DNA with KS, we examined in total 42 cases of vascular neoplasms of endothelial derivation using nested polymerase chain reaction (PCR) for the presence of a 233-bp segment of this KSHV/HHV8 on paraffin-embedded specimens. In our investigation, we added an additional step to conventional PCR protocol that uses UV light to pretreat all the PCR regeants except Taq DNA polymerase and the target DNA to eliminate the false positives caused by trace contamination. All 15 cases of typical KS, both AIDS and non-AIDS related, as well as 4 cases of atypical vascular tumors suspicious of KS, were positive for this KSHV/HHV8 DNA sequence. The remaining 23 cases of hemangioproliferative disorders other than KS, including angiosarcoma, capillary hemangioma, angiolymphoid hyperplasia with eosinophilia, epithelioid hemangioma, histiocytoid hemangioma, hemangioendothelioma, and microvenous hemangioma, were negative for HHV8. These results confirm the previous observation that KSHV/HHV8 is specific for KS within hemangioproliferative cutaneous disorders, and PCR for detection of KSHV/HHV8 might be used as an additional diagnostic tool in distinguishing KS.
最近，一种名为卡波西肉瘤相关疱疹病毒（KSHV）或人类疱疹病毒8型（HHV8）的新型疱疹病毒样DNA序列已从几乎所有卡波西肉瘤（KS）病例中分离出来。除KS外，在大多数良性和恶性皮肤血管增生性疾病中均未发现该病毒。为了进一步验证这种新型病毒DNA与KS关联的特异性，我们使用巢式聚合酶链反应（PCR）检测了42例内皮源性血管肿瘤石蜡包埋标本中是否存在这种KSHV/HHV8的233bp片段。在我们的研究中，我们在传统PCR方案中增加了一个额外步骤，即使用紫外线预处理除Taq DNA聚合酶和靶DNA之外的所有PCR试剂，以消除微量污染导致的假阳性。15例典型KS病例，包括艾滋病相关和非艾滋病相关的，以及4例疑似KS的非典型血管肿瘤病例，该KSHV/HHV8 DNA序列均呈阳性。其余23例非KS的血管增生性疾病，包括血管肉瘤、毛细血管瘤、嗜酸性粒细胞增多性血管淋巴样增生、上皮样血管瘤、组织细胞样血管瘤、血管内皮瘤和微静脉血管瘤，HHV8均为阴性。这些结果证实了先前的观察结果，即KSHV/HHV8在皮肤血管增生性疾病中对KS具有特异性，检测KSHV/HHV8的PCR可作为区分KS的一种辅助诊断工具。
