编码分泌信号蛋白的肠致病性大肠杆菌毒力基因对于调节Caco-2细胞电解质转运至关重要。
Enteropathogenic Escherichia coli virulence genes encoding secreted signalling proteins are essential for modulation of Caco-2 cell electrolyte transport.
作者信息
Collington G K, Booth I W, Donnenberg M S, Kaper J B, Knutton S
机构信息
Institute of Child Health, University of Birmingham, Birmingham B4 6NH, United Kingdom.
出版信息
Infect Immun. 1998 Dec;66(12):6049-53. doi: 10.1128/IAI.66.12.6049-6053.1998.
Abstract
The pathophysiology of enteropathogenic Escherichia coli (EPEC) diarrhea remains uncertain. In vitro, EPEC stimulates a rapid increase in short-circuit current (Isc) across Caco-2 cell monolayers coincident with intimate attaching and effacing (A/E) bacterial adhesion. This study has examined the roles of specific EPEC virulence proteins in this Isc response. EPEC genes encoding EspA, EspB, and EspD, essential for signal transduction in host cells and A/E activity, were also required for modulation of Caco-2 electrolyte transport.
摘要
肠致病性大肠杆菌（EPEC）腹泻的病理生理学仍不明确。在体外，EPEC刺激跨Caco-2细胞单层的短路电流（Isc）迅速增加，同时伴有紧密黏附和消除（A/E）细菌黏附。本研究检测了特定EPEC毒力蛋白在这种Isc反应中的作用。编码EspA、EspB和EspD的EPEC基因是宿主细胞信号转导和A/E活性所必需的，也是调节Caco-2电解质转运所必需的。
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本文引用的文献
1
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Mol Microbiol. 1998 Apr;28(1):1-4. doi: 10.1046/j.1365-2958.1998.00783.x.
2
A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells.一种与肠致病性大肠杆菌 EspA 相关的新型表面细胞器，参与蛋白质转运至上皮细胞。
EMBO J. 1998 Apr 15;17(8):2166-76. doi: 10.1093/emboj/17.8.2166.
3
Rapid modulation of electrolyte transport in Caco-2 cell monolayers by enteropathogenic Escherichia coli (EPEC) infection.肠致病性大肠杆菌（EPEC）感染对Caco-2细胞单层中电解质转运的快速调节作用。
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4
Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells.肠道致病性大肠杆菌（EPEC）将其紧密黏附受体转移至哺乳动物细胞中。
Cell. 1997 Nov 14;91(4):511-20. doi: 10.1016/s0092-8674(00)80437-7.
5
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Infect Immun. 1997 Jul;65(7):2606-12. doi: 10.1128/iai.65.7.2606-2612.1997.
6
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Infect Immun. 1997 Jul;65(7):2528-36. doi: 10.1128/iai.65.7.2528-2536.1997.
7
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8
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