Haas E S, Banta A B, Harris J K, Pace N R, Brown J W
Department of Microbiology, North Carolina State University, Raleigh 27695, USA.
Nucleic Acids Res. 1996 Dec 1;24(23):4775-82. doi: 10.1093/nar/24.23.4775.
The sequences and structures of RNase P RNAs of some Gram-positive bacteria, e.g. Bacillus subtilis, are very different than those of other bacteria. In order to expand our understanding of the structure and evolution of RNase P RNA in Gram-positive bacteria, gene sequences encoding RNase P RNAs from 10 additional species from this evolutionary group have been determined, doubling the number of sequences available for comparative analysis. The enlarged data set allows refinement of the secondary structure model of these unusual RNase P RNAs and the identification of potential tertiary interactions between P10.1 and L12, and between L5.1 and L15.1. The newly-obtained sequences suggest that RNase P RNA underwent an abrupt, dramatic restructuring in the ancestry of the low-G+C Gram-positive bacteria after the divergence of the branches leading to the 'Clostridia and relatives' and the remaining low-G+C Gram-positive species. The unusual structures of the RNase P RNAs of Mycoplasma hyopneumoniae and M.floccularre are apparently derived from RNAs with Bacillus-like structure rather than from intermediate, partially restructured ancestral RNAs. The structure of the RNase P RNA from the photosynthetic Heliobacillus mobilis supports the relationship of this specie with Bacillus and Staphylococcus rather than the 'Clostridia and relatives' as suggested by the sequences of their small-subunit ribosomal RNAs.
一些革兰氏阳性细菌（如枯草芽孢杆菌）的核糖核酸酶P（RNase P）RNA的序列和结构与其他细菌的序列和结构有很大不同。为了拓展我们对革兰氏阳性细菌中RNase P RNA结构和进化的理解，我们测定了来自该进化组另外10个物种的编码RNase P RNA的基因序列，使可用于比较分析的序列数量增加了一倍。扩大后的数据集有助于完善这些特殊RNase P RNA的二级结构模型，并确定P10.1与L12之间以及L5.1与L15.1之间潜在的三级相互作用。新获得的序列表明，在导致“梭菌及其亲缘种”的分支与其余低G+C革兰氏阳性物种分化之后，RNase P RNA在低G+C革兰氏阳性细菌的祖先中经历了一次突然的、剧烈的结构重组。猪肺炎支原体和絮状支原体的RNase P RNA的异常结构显然源自具有芽孢杆菌样结构的RNA，而不是源自中间的、部分重组的祖先RNA。光合游动太阳杆菌的RNase P RNA结构支持了该物种与芽孢杆菌和葡萄球菌的关系，而不是其小亚基核糖体RNA序列所表明的与“梭菌及其亲缘种”的关系。
