Hiraishi A, Nagashima K V, Matsuura K, Shimada K, Takaichi S, Wakao N, Katayama Y
Department of Ecological Engineering, Toyohashi University of Technology, Japan.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1389-98. doi: 10.1099/00207713-48-4-1389.
Phylogenetic analyses based on 16S rDNA sequences and genomic DNA-DNA relatedness showed that the sulphur-oxidizing facultative chemolithotroph Thiobacillus acidophilus was closely related to members of the genus Acidiphilium, which is a group of strictly aerobic, heterotrophic acidophiles now categorized into aerobic photosynthetic bacteria. Lipophilic pigment analyses revealed that zinc-chelated bacteriochlorophyll a and carotenoids occurred in appreciable amounts in T. acidophilus and all established species of the genus Acidiphilium. PCR experiments showed that T. acidophilus as well as Acidiphilium species contained puf genes, encoding the photosynthetic reaction centre proteins and the core light-harvesting complex of the purple bacteria. There were high similarities between T. acidophilus and Acidiphilium species in the primary structure of their reaction centre proteins deduced from the nucleotide sequence data. The phylogenetic tree of the reaction centre proteins was in agreement with the 16S rDNA sequence-based phylogenetic tree in the relationship between T. acidophilus and Acidiphilium species and between the Acidiphilium cluster and other purple photosynthetic bacteria. Based on these results, together with previous phylogenetic and phenotypic information, it is proposed to reclassify T. acidophilus (Guay and Silver) Harrison 1983 as Acidiphilium acidophilum comb. nov. The type strain is ATCC 27807T (= DSM 700T).
基于16S rDNA序列和基因组DNA - DNA相关性的系统发育分析表明，硫氧化兼性化能自养菌嗜酸硫杆菌与嗜酸菌属的成员密切相关，嗜酸菌属是一组严格需氧的异养嗜酸菌，现在被归类为需氧光合细菌。亲脂性色素分析显示，锌螯合细菌叶绿素a和类胡萝卜素在嗜酸硫杆菌和嗜酸菌属所有已确定的物种中大量存在。PCR实验表明，嗜酸硫杆菌以及嗜酸菌属物种都含有puf基因，该基因编码光合反应中心蛋白和紫色细菌的核心光捕获复合体。从核苷酸序列数据推导得出，嗜酸硫杆菌和嗜酸菌属物种的反应中心蛋白一级结构具有高度相似性。在嗜酸硫杆菌与嗜酸菌属物种之间以及嗜酸菌属簇与其他紫色光合细菌之间的关系上，反应中心蛋白的系统发育树与基于16S rDNA序列的系统发育树一致。基于这些结果，结合先前的系统发育和表型信息，建议将嗜酸硫杆菌（Guay和Silver）Harrison 1983重新分类为嗜酸嗜酸菌（Acidiphilium acidophilum）新组合。模式菌株为ATCC 27807T（= DSM 700T）。
