Kataoka H
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
J Chromatogr B Biomed Sci Appl. 1998 Oct 9;717(1-2):247-62. doi: 10.1016/s0378-4347(97)00628-2.
The analysis of lipoic acid and related compounds, such as its reduced form dihydrolipoic acid, its amide form lipoamide and other analogues, in biological and food samples is important in biochemistry, nutritional and clinical chemistry. This review summarizes the chromatographic methods for the determination of lipoic acid and related compounds, and their applications to various samples such as bacteria, tissues, drugs and food. Gas chromatographic methods with flame ionization detection and flame photometric detection are commonly used for the quantification of lipoic acid present as its protein-bound form, after acid or base hydrolysis of these samples. High-performance liquid chromatographic methods with ultraviolet, fluorescence and electrochemical detection are mainly used for the determination of free lipoic acid and related compounds, such as dihydrolipoic acid, lipoamide and other analogues. Moreover, gas chromatography-mass spectrometry and capillary electrophoresis methods are also developed.
对生物和食品样本中的硫辛酸及其相关化合物（如还原形式的二氢硫辛酸、酰胺形式的硫辛酰胺和其他类似物）进行分析，在生物化学、营养和临床化学领域具有重要意义。本综述总结了用于测定硫辛酸及其相关化合物的色谱方法，以及它们在各种样本（如细菌、组织、药物和食品）中的应用。火焰离子化检测和火焰光度检测的气相色谱方法通常用于在对这些样本进行酸或碱水解后，对以蛋白质结合形式存在的硫辛酸进行定量。配备紫外、荧光和电化学检测的高效液相色谱方法主要用于测定游离硫辛酸及其相关化合物，如二氢硫辛酸、硫辛酰胺和其他类似物。此外，气相色谱 - 质谱联用和毛细管电泳方法也得到了发展。
