成团泛菌T71中没食子酸脱羧酶的纯化及特性分析
Purification and characterization of gallic acid decarboxylase from pantoea agglomerans T71.
作者信息
Zeida M, Wieser M, Yoshida T, Sugio T, Nagasawa T
机构信息
Department of Biological Function & Genetic Resources Sciences, Faculty of Agriculture, Okayama University, Tsushima Naka, Okayama 700-11, Japan.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):4743-7. doi: 10.1128/AEM.64.12.4743-4747.1998.
Abstract
Oxygen-sensitive gallic acid decarboxylase from Pantoea (formerly Enterobacter) agglomerans T71 was purified from a cell extract after stabilization by reducing agents. This enzyme has a molecular mass of approximately 320 kDa and consists of six identical subunits. It is highly specific for gallic acid. Gallic acid decarboxylase is unique among similar decarboxylases in that it requires iron as a cofactor, as shown by plasma emission spectroscopy (which revealed an iron content of 0.8 mol per mol of enzyme subunit), spectrophotometric analysis (absorption shoulders at 398 and 472 nm), and inhibition of the enzyme activity by 2,2'-bipyridyl, o-phenanthroline, and EDTA. Another interesting feature of this strain is the fact that it contains a tannase, which is used together with the gallic acid decarboxylase in a two-enzyme resting cell bioconversion to synthesize valuable pyrogallol from readily available tannic acid.
摘要
从成团泛菌（以前称为聚团肠杆菌）T71中提取的对氧敏感的没食子酸脱羧酶，在经过还原剂稳定处理后从细胞提取物中纯化得到。这种酶的分子量约为320 kDa，由六个相同的亚基组成。它对没食子酸具有高度特异性。没食子酸脱羧酶在类似的脱羧酶中是独特的，因为它需要铁作为辅因子，这通过等离子体发射光谱法（显示每摩尔酶亚基的铁含量为0.8摩尔）、分光光度分析（在398和472 nm处有吸收峰）以及2,2'-联吡啶、邻菲罗啉和EDTA对酶活性的抑制作用得以证明。该菌株的另一个有趣特征是它含有一种单宁酶，在双酶静息细胞生物转化中，单宁酶与没食子酸脱羧酶一起用于从容易获得的单宁酸合成有价值的焦性没食子酸。
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本文引用的文献
1
Enzymatic gallic acid esterification.酶促没食子酸酯化反应。
Biotechnol Bioeng. 1985 Feb;27(2):124-7. doi: 10.1002/bit.260270203.
2
Isolation, Purification, and Some Properties of Penicillium chrysogenum Tannase.桔青霉单宁酶的分离纯化及部分性质研究
Appl Environ Microbiol. 1983 Aug;46(2):525-7. doi: 10.1128/aem.46.2.525-527.1983.
3
Production and application of tannin acyl hydrolase: state of the art.单宁酰基水解酶的生产与应用：现状
Adv Appl Microbiol. 1997;44:215-60. doi: 10.1016/s0065-2164(08)70463-5.
4
Biochemical characteristics of Enterobacter agglomerans and related strains found in buckwheat seeds.在荞麦种子中发现的成团肠杆菌及相关菌株的生化特性。
Int J Food Microbiol. 1996 Jul;30(3):243-53. doi: 10.1016/0168-1605(96)00949-x.
5
Purification and characterization of an oxygen-sensitive, reversible 3,4-dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum.来自羟基苯甲酸梭菌的一种对氧敏感的、可逆的3,4-二羟基苯甲酸脱羧酶的纯化及特性研究
J Bacteriol. 1996 Jun;178(12):3539-43. doi: 10.1128/jb.178.12.3539-3543.1996.
6
Identification of the active-site peptide of 2,3-dihydroxybenzoic acid decarboxylase from Aspergillus oryzae.米曲霉2,3-二羟基苯甲酸脱羧酶活性位点肽段的鉴定
Biochim Biophys Acta. 1996 Apr 16;1293(2):191-200. doi: 10.1016/0167-4838(95)00242-1.
7
An investigation of gram-negative tannin-protein complex degrading bacteria in fecal flora of various mammals.
J Vet Med Sci. 1995 Oct;57(5):921-6. doi: 10.1292/jvms.57.921.
8
Initial steps in the anaerobic degradation of 3,4,5-trihydroxybenzoate by Eubacterium oxidoreducens: characterization of mutants and role of 1,2,3,5-tetrahydroxybenzene.氧化还原真杆菌对3,4,5-三羟基苯甲酸进行厌氧降解的初始步骤：突变体的表征及1,2,3,5-四羟基苯的作用
J Bacteriol. 1993 Feb;175(3):669-73. doi: 10.1128/jb.175.3.669-673.1993.
9
Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum.来自羟基苯甲酸梭菌的一种对氧敏感的可逆4-羟基苯甲酸脱羧酶的纯化与特性分析
Eur J Biochem. 1995 Apr 1;229(1):77-82. doi: 10.1111/j.1432-1033.1995.tb20440.x.
10
2,3-Dihydroxybenzoic acid decarboxylase from Aspergillus niger. A novel decarboxylase.来自黑曲霉的2,3-二羟基苯甲酸脱羧酶。一种新型脱羧酶。
Eur J Biochem. 1995 May 15;230(1):104-10.
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