Janssen PH
Max-Planck-Institut fur terrestrische Mikrobiologie, D-35043 Marburg, Germany.
Appl Environ Microbiol. 1998 Dec;64(12):4830-3. doi: 10.1128/AEM.64.12.4830-4833.1998.
Strain VeGlc2, an anaerobic ultramicrobacterium belonging to the Verrucomicrobiales lineage of bacterial descent, fermented glucose to acetate, propionate, succinate, and CO2. The distribution of radiolabel in the fermentation end products produced from position-labelled glucose and in vitro measurements of enzyme activities in crude cell extracts prepared from glucose-grown cells showed that glucose was metabolized via the Embden-Meyerhof-Parnas pathway. The 6-phosphofructokinase (EC 2.7.1.90) activity required pyrophosphate as the phosphoryl donor, and ATP could not replace pyrophosphate. The other enzyme activities were those of a classical Embden-Meyerhof-Parnas pathway. 14CO2 was incorporated into propionate and succinate, suggesting that a carboxylation reaction rather than a transcarboxylation reaction was involved in the reductive pathway leading to succinate and propionate. Difference spectra showed that a type b cytochrome was present, which could be involved in electron transport in the reductive pathway.
菌株VeGlc2是一种厌氧超微细菌，属于疣微菌门细菌谱系，可将葡萄糖发酵为乙酸盐、丙酸盐、琥珀酸盐和二氧化碳。由位置标记的葡萄糖产生的发酵终产物中放射性标记的分布，以及从葡萄糖培养的细胞制备的粗细胞提取物中酶活性的体外测量结果表明，葡萄糖通过糖酵解途径进行代谢。6-磷酸果糖激酶（EC 2.7.1.90）活性需要焦磷酸作为磷酰基供体，ATP不能替代焦磷酸。其他酶活性是经典糖酵解途径的酶活性。14CO2被掺入丙酸盐和琥珀酸盐中，这表明导致琥珀酸盐和丙酸盐的还原途径涉及羧化反应而非转羧化反应。差示光谱表明存在b型细胞色素，其可能参与还原途径中的电子传递。
