用于在生物降解实验中对恶臭假单胞菌F1、伯克霍尔德氏菌菌株JS150和枯草芽孢杆菌ATCC 7003进行计数的物种特异性寡核苷酸。
Species-specific oligonucleotides for enumeration of Pseudomonas putida F1, Burkholderia sp. strain JS150, and Bacillus subtilis ATCC 7003 in biodegradation experiments.
作者信息
DuTeau N M, Rogers J D, Bartholomay C T, Reardon K F
机构信息
Departments of Microbiology, Center for Environmental Toxicology and Technology, Colorado State University, Fort Collins, Colorado 80523, USA.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):4994-9. doi: 10.1128/AEM.64.12.4994-4999.1998.
Abstract
Species-specific sequences were identified within the V4 variable region of 16S rRNA of two bacterial species capable of aromatic hydrocarbon metabolism, Pseudomonas putida F1 and Burkholderia sp. strain JS150, and a third, Bacillus subtilis ATCC 7003, that can function as a secondary degrader. Fluorescent in situ hybridization (FISH) with species-specific oligonucleotides was used for direct counting of these species throughout a phenol biodegradation experiment in batch culture. Traditional differential plate counting methods could not be used due to the similar metabolism and interactions of the primary degraders and difficulties in selecting secondary degraders in mixed culture. In contrast, the FISH method provided reliable quantitative results without interference from those factors.
摘要
在两种能够进行芳烃代谢的细菌物种——恶臭假单胞菌F1和伯克霍尔德氏菌属菌株JS150，以及第三种能够作为二级降解菌的枯草芽孢杆菌ATCC 7003的16S rRNA的V4可变区内，鉴定出了物种特异性序列。在分批培养的苯酚生物降解实验中，使用与物种特异性寡核苷酸的荧光原位杂交（FISH）技术对这些物种进行直接计数。由于初级降解菌的代谢和相互作用相似，以及在混合培养中选择二级降解菌存在困难，传统的差异平板计数方法无法使用。相比之下，FISH方法提供了可靠的定量结果，不受这些因素的干扰。
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