Grimm D, Merkert H, Ludwig W, Schleifer K H, Hacker J, Brand B C
Institut für Molekulare Infektionsbiologie, Würzburg, Germany.
Appl Environ Microbiol. 1998 Jul;64(7):2686-90. doi: 10.1128/AEM.64.7.2686-2690.1998.
Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of L. pneumophila from other Legionella species without cultivation. The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of L. pneumophila, five different Legionella spp. and three nonlegionella species as reference strains. Furthermore, L. pneumophila cells could be easily distinguished from Legionella micdadei and Pseudomonas aeruginosa cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan Acanthamoeba castellanii.
基于比较序列分析，我们设计了一种与嗜肺军团菌16S rRNA区域互补的寡核苷酸探针，该探针可在无需培养的情况下将嗜肺军团菌与其他军团菌属物种区分开来。通过对总共六个菌株的四个血清群的嗜肺军团菌、五个不同的军团菌属物种以及三个非军团菌物种作为参考菌株进行原位杂交，测试了新探针LEGPNE1的特异性。此外，在用探针LEGPNE1、LEG705和EUB338对原生动物卡氏棘阿米巴进行感染后，通过原位杂交可以轻松地将嗜肺军团菌细胞与米克戴德军团菌和铜绿假单胞菌细胞区分开来。
