嗜肺军团菌的特异性检测：一种新型16S rRNA靶向寡核苷酸探针的构建
Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe.
作者信息
Grimm D, Merkert H, Ludwig W, Schleifer K H, Hacker J, Brand B C
机构信息
Institut für Molekulare Infektionsbiologie, Würzburg, Germany.
出版信息
Appl Environ Microbiol. 1998 Jul;64(7):2686-90. doi: 10.1128/AEM.64.7.2686-2690.1998.
Abstract
Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of L. pneumophila from other Legionella species without cultivation. The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of L. pneumophila, five different Legionella spp. and three nonlegionella species as reference strains. Furthermore, L. pneumophila cells could be easily distinguished from Legionella micdadei and Pseudomonas aeruginosa cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan Acanthamoeba castellanii.
摘要
基于比较序列分析，我们设计了一种与嗜肺军团菌16S rRNA区域互补的寡核苷酸探针，该探针可在无需培养的情况下将嗜肺军团菌与其他军团菌属物种区分开来。通过对总共六个菌株的四个血清群的嗜肺军团菌、五个不同的军团菌属物种以及三个非军团菌物种作为参考菌株进行原位杂交，测试了新探针LEGPNE1的特异性。此外，在用探针LEGPNE1、LEG705和EUB338对原生动物卡氏棘阿米巴进行感染后，通过原位杂交可以轻松地将嗜肺军团菌细胞与米克戴德军团菌和铜绿假单胞菌细胞区分开来。
相似文献
1
Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe.嗜肺军团菌的特异性检测：一种新型16S rRNA靶向寡核苷酸探针的构建
Appl Environ Microbiol. 1998 Jul;64(7):2686-90. doi: 10.1128/AEM.64.7.2686-2690.1998.
2
In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy.使用16S rRNA靶向寡核苷酸探针和共聚焦激光扫描显微镜对军团菌科进行原位鉴定。
Microbiology (Reading). 1995 Jan;141 ( Pt 1):29-39. doi: 10.1099/00221287-141-1-29.
3
Development of 18S rRNA-targeted oligonucleotide probes for specific detection of Hartmannella and Naegleria in Legionella-positive environmental samples.用于在军团菌阳性环境样本中特异性检测哈特曼氏阿米巴属和耐格里属的18S rRNA靶向寡核苷酸探针的开发。
Syst Appl Microbiol. 2001 Apr;24(1):76-82. doi: 10.1078/0723-2020-00017.
4
5
Development of conventional and real-time NASBA for the detection of Legionella species in respiratory specimens.用于检测呼吸道标本中军团菌属的传统和实时核酸序列扩增技术的开发。
J Microbiol Methods. 2006 Dec;67(3):408-15. doi: 10.1016/j.mimet.2006.04.012. Epub 2006 May 30.
6
Use of the dnaJ gene for the detection and identification of all Legionella pneumophila serogroups and description of the primers used to detect 16S rDNA gene sequences of major members of the genus Legionella.利用dnaJ基因检测和鉴定所有嗜肺军团菌血清型，并描述用于检测军团菌属主要成员16S rDNA基因序列的引物。
Microbiol Immunol. 2003;47(11):859-69. doi: 10.1111/j.1348-0421.2003.tb03452.x.
7
Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis.通过双色实时荧光定量PCR和熔解曲线分析直接检测和鉴别临床标本中的嗜肺军团菌及其他军团菌属细菌。
J Clin Microbiol. 2002 Oct;40(10):3814-7. doi: 10.1128/JCM.40.10.3814-3817.2002.
8
Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii.卡氏棘阿米巴对存活但不可培养的嗜肺军团菌费城株JR32的复苏作用
Appl Environ Microbiol. 1997 May;63(5):2047-53. doi: 10.1128/aem.63.5.2047-2053.1997.
9
Targeting single-nucleotide polymorphisms in the 16S rRNA gene to detect and differentiate Legionella pneumophila and non-Legionella pneumophila species.靶向16S rRNA基因中的单核苷酸多态性以检测和区分嗜肺军团菌和非嗜肺军团菌物种。
Arch Microbiol. 2016 Aug;198(6):591-4. doi: 10.1007/s00203-016-1228-2. Epub 2016 Apr 25.
10
Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing.利用靶向16S rRNA基因的实时PCR鉴定和区分嗜肺军团菌及其他军团菌属细菌，并通过mip测序进行菌种鉴定。
Appl Environ Microbiol. 2006 Sep;72(9):6394-8. doi: 10.1128/AEM.02839-05.
引用本文的文献
1
Symbiont-Mediated Defense against Legionella pneumophila in Amoebae.共生体介导的对粘细菌的防御作用。
mBio. 2019 May 14;10(3):e00333-19. doi: 10.1128/mBio.00333-19.
2
Effect of Common Drinking Water Disinfectants, Chlorine and Heat, on Free Legionella and Amoebae-Associated Legionella.常用饮用水消毒剂（氯和热）对游离嗜肺军团菌及与变形虫相关的嗜肺军团菌的影响
PLoS One. 2015 Aug 4;10(8):e0134726. doi: 10.1371/journal.pone.0134726. eCollection 2015.
3
Spatial arrangement of legionella colonies in intact biofilms from a model cooling water system.来自模型冷却水系统的完整生物膜中军团菌菌落的空间排列。
Microbiol Insights. 2013 Jul 4;6:49-57. doi: 10.4137/MBI.S12196. eCollection 2013.
4
Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain.西班牙应用 ScanVIT-Legionella™ 方法检测环境水样中的军团菌属。
Indian J Microbiol. 2013 Jun;53(2):142-8. doi: 10.1007/s12088-013-0363-6. Epub 2013 Feb 3.
5
Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.利用肽核酸靶向物种特异性低亲和力16S rRNA结合位点以检测生物膜中的军团菌。
Appl Environ Microbiol. 2006 Aug;72(8):5453-62. doi: 10.1128/AEM.02918-05.
6
Pontiac fever: an operational definition for epidemiological studies.庞蒂亚克热：流行病学研究的操作定义
BMC Public Health. 2006 Apr 28;6:112. doi: 10.1186/1471-2458-6-112.
7
Impact of non-Legionella bacteria on the uptake and intracellular replication of Legionella pneumophila in Acanthamoeba castellanii and Naegleria lovaniensis.非嗜肺军团菌对嗜肺军团菌在卡氏棘阿米巴和洛万内格利氏阿米巴中摄取及细胞内复制的影响。
Microb Ecol. 2005 Nov;50(4):536-49. doi: 10.1007/s00248-005-0258-0. Epub 2005 Dec 15.
8
Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.通过免疫磁珠纯化和dotA基因的实时PCR扩增对水样中嗜肺军团菌进行定量检测。
Appl Environ Microbiol. 2005 Jul;71(7):3433-41. doi: 10.1128/AEM.71.7.3433-3441.2005.
9
Intracellular proliferation of Legionella pneumophila in Hartmannella vermiformis in aquatic biofilms grown on plasticized polyvinyl chloride.嗜肺军团菌在软质聚氯乙烯上生长的水生生物膜中的蠕虫状哈特曼氏阿米巴中进行细胞内增殖。
Appl Environ Microbiol. 2004 Nov;70(11):6826-33. doi: 10.1128/AEM.70.11.6826-6833.2004.
10
Detection and differentiation of chlamydiae by fluorescence in situ hybridization.通过荧光原位杂交技术检测和鉴别衣原体。
Appl Environ Microbiol. 2002 Aug;68(8):4081-9. doi: 10.1128/AEM.68.8.4081-4089.2002.
本文引用的文献
1
Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii.卡氏棘阿米巴对存活但不可培养的嗜肺军团菌费城株JR32的复苏作用
Appl Environ Microbiol. 1997 May;63(5):2047-53. doi: 10.1128/aem.63.5.2047-2053.1997.
2
Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods.通过聚合酶链反应、直接荧光抗体法和平板培养法检测污水和海水中的军团菌属。
Appl Environ Microbiol. 1993 Nov;59(11):3618-24. doi: 10.1128/aem.59.11.3618-3624.1993.
3
Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization.通过rRNA原位杂交推断小鼠大肠中大肠杆菌的空间分布。
Infect Immun. 1994 Nov;62(11):5191-4. doi: 10.1128/iai.62.11.5191-5194.1994.
4
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.无需培养的单个微生物细胞的系统发育鉴定和原位检测。
Microbiol Rev. 1995 Mar;59(1):143-69. doi: 10.1128/mr.59.1.143-169.1995.
5
In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy.使用16S rRNA靶向寡核苷酸探针和共聚焦激光扫描显微镜对军团菌科进行原位鉴定。
Microbiology (Reading). 1995 Jan;141 ( Pt 1):29-39. doi: 10.1099/00221287-141-1-29.
6
Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.一种针对不动杆菌属的rRNA靶向寡核苷酸探针的开发及其在活性污泥原位监测中的应用。
Appl Environ Microbiol. 1994 Mar;60(3):792-800. doi: 10.1128/aem.60.3.792-800.1994.
7
Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes.军团菌病细菌（嗜肺军团菌）在人类单核细胞内进行细胞内增殖。
J Clin Invest. 1980 Sep;66(3):441-50. doi: 10.1172/JCI109874.
8
Legionella pneumophila in cooling water systems. Report of a survey of cooling towers in London and a pilot trial of selected biocides.冷却水系中的嗜肺军团菌。伦敦冷却塔调查及选定杀生剂的试点试验报告。
J Hyg (Lond). 1982 Jun;88(3):369-81. doi: 10.1017/s0022172400070248.
9
A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
J Mol Biol. 1969 May 14;41(3):459-72. doi: 10.1016/0022-2836(69)90288-5.
10
A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs.基于豚鼠实验性气溶胶感染对两株嗜肺军团菌毒力的比较。
J Hyg (Lond). 1985 Aug;95(1):29-38. doi: 10.1017/s0022172400062252.
Zotero 插件