Fimland G, Jack R, Jung G, Nes I F, Nissen-Meyer J
Department of Biochemistry, University of Oslo, Oslo, Norway.
Appl Environ Microbiol. 1998 Dec;64(12):5057-60. doi: 10.1128/AEM.64.12.5057-5060.1998.
A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A, however, was also significantly inhibited by this fragment, although not as greatly as pediocin PA-1. This is consistent with the fact that enterocin A contains the longest continuous sequence identical to that of pediocin PA-1 in the region spanned by the fragment. The fragment inhibited pediocin PA-1 to a much greater extent than did the other 29 possible 15-mer fragments that span pediocin PA-1. The results suggest that the fragment-by interacting with the target cells and/or pediocin PA-1-interferes specifically with pediocin-target cell interaction.
来源于片球菌素PA-1（第20位氨基酸残基至第34位氨基酸残基）的一个15聚体肽片段可特异性抑制片球菌素PA-1的杀菌活性。该片段对片球菌素样细菌素片球菌素P、亮菌素A和弯曲菌素A的抑制程度，远不及对片球菌素PA-1的抑制程度。然而，肠球菌素A也受到该片段的显著抑制，尽管程度不如片球菌素PA-1。这与肠球菌素A在该片段所覆盖区域含有与片球菌素PA-1最长连续相同序列这一事实相符。该片段对片球菌素PA-1的抑制程度，远高于跨越片球菌素PA-1的其他29个可能的15聚体片段。结果表明，该片段通过与靶细胞和/或片球菌素PA-1相互作用，特异性干扰片球菌素与靶细胞的相互作用。
