艾滋病患者宫颈上皮内病变中HPV各型的非同位素原位杂交

Non-isotopic in situ hybridization of HPV types in cervical intraepithelial lesions in patients with AIDS.

作者信息

Calore E E, Calore N M, Cavaliere M J

机构信息

Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil.

出版信息

Arch Gynecol Obstet. 1998;262(1-2):75-9. doi: 10.1007/s004040050231.

DOI:10.1007/s004040050231

PMID:9836004

Abstract

Human papilloma viruses (HPVs), particularly types 16 and 18 have a key role in the development of preneoplastic and neoplastic lesions of the uterine cervix. We studied, by non isotopic in situ hybridization using probes to HPV 6, 11, 16 and 18, cervical biopsies from AIDS patients with condilomata or cervical intraepithelial neoplasia. There were 32 biopsies which showed low-grade cervical intraepithelial neoplasia (Lo-CIN); 5 biopsies showed high-grade cervical intraepithelial neoplasia (Hi-CIN). Of 32 Lo-CIN biopsies, 18 (56.3%) were positive for HPV; 7 for HPV 6 and/or 11 (21.9%), 11 for HPV 16 and/or 18 (34.4%) and one for HPV 6 and 18. Of 5 Hi-CIN biopsies 3 were positive for HPV: one for HPV 6 and 2 for HPV 16 or 18. The total positivity was 56.8% (21/37). This result was similar to those obtained by various other authors studying the general population.

摘要

人乳头瘤病毒（HPV），尤其是16型和18型，在子宫颈癌前病变和肿瘤性病变的发生发展中起关键作用。我们使用针对HPV 6、11、16和18型的探针，通过非同位素原位杂交技术，对患有尖锐湿疣或宫颈上皮内瘤变的艾滋病患者的宫颈活检样本进行了研究。有32份活检样本显示为低级别宫颈上皮内瘤变（Lo-CIN）；5份活检样本显示为高级别宫颈上皮内瘤变（Hi-CIN）。在32份Lo-CIN活检样本中，18份（56.3%）HPV呈阳性；7份HPV 6和/或11型阳性（21.9%），11份HPV 16和/或18型阳性（34.4%），1份HPV 6和18型阳性。在5份Hi-CIN活检样本中，3份HPV呈阳性：1份HPV 6型阳性，2份HPV 16或18型阳性。总阳性率为56.8%（21/37）。该结果与其他研究普通人群的作者所获得的结果相似。