Osterlund M T, Deng X W
Department of Molecular Cell and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Plant J. 1998 Oct;16(2):201-8. doi: 10.1046/j.1365-313x.1998.00290.x.
The subcellular localization of COP1, a key photomorphogenic repressor, is regulated by light in Arabidopsis seedlings. Photoreceptor loss-of-function mutants and dominant gain-of-function overexpression transgenes were both used to analyze the influences of the three photoreceptors, phyA, phyB, and CRY1, on the light-regulated subcellular localization of COP1. Through a semiquantitative analysis of the nuclear abundance of GUS-COP1 in the various genetic backgrounds, the specific roles of the individual photoreceptors have been established. The data suggest that multiple photoreceptors influence the light-regulated subcellular localization of COP1 in white light. Under specific wavelengths of light, phyA, phyB, and CRY1 each play critical roles in mediating far-red, red, and blue light signals, respectively. Our data also support an interdependency between CRY1 and the phytochromes in mediating the light-regulated subcellular localization of COP1 and thus seedling development.
COP1是一种关键的光形态建成抑制因子，在拟南芥幼苗中，其亚细胞定位受光调控。利用光受体功能缺失突变体和显性功能获得性过表达转基因，分析了三种光受体phyA、phyB和CRY1对COP1光调控亚细胞定位的影响。通过对不同遗传背景下GUS-COP1核丰度的半定量分析，确定了单个光受体的具体作用。数据表明，多种光受体在白光下影响COP1的光调控亚细胞定位。在特定波长的光下，phyA、phyB和CRY1分别在介导远红光、红光和蓝光信号中发挥关键作用。我们的数据还支持CRY1与光敏色素之间在介导COP1的光调控亚细胞定位从而影响幼苗发育方面存在相互依赖性。
