Oshiro Y, Chaturvedi V, Hayden D, Nazeer T, Johnson M, Johnston D A, Ordóñez N G, Ayala A G, Czerniak B
Department of Pathology, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Cancer. 1998 Dec 1;83(11):2324-34. doi: 10.1002/(sici)1097-0142(19981201)83:11<2324::aid-cncr12>3.0.co;2-u.
p53 is a major tumor suppressor gene that has been implicated in the biology of a variety of human neoplasms, including some that affect the skeleton. Recent studies based on small numbers of cases have shown that overexpression or alteration of the p53 gene is frequently present in high grade, clinically aggressive chondrosarcomas of bone. In this study, the authors addressed the relation between overexpression and alteration of the p53 gene and the clinical aggressiveness of chondrosarcoma in a large series of patients for whom long term follow-up data were available.
The authors analyzed the expression and/or alteration of the p53 gene in 158 cases of chondrosarcoma of bone using immunohistochemistry, single-strand conformation polymorphism, and direct sequencing. They then related the findings to various clinicopathologic parameters and long term follow-up data.
The presence of overexpression and/or structural alterations of the p53 gene was documented in 38.1% of chondrosarcomas of bone. A statistically significant correlation was observed between overexpression or alteration of the p53 gene and both the histologic grade of the tumor and the presence of metastasis. The probability of local recurrence free, metastasis free, and overall survival was significantly higher for patients with no overexpression or alteration of p53 than for patients with p53 overexpression or alteration.
Overexpression or alteration of the p53 gene is an important predictor of aggressive clinical behavior in chondrosarcoma of bone.
p53是一种主要的肿瘤抑制基因，与多种人类肿瘤的生物学行为相关，包括一些影响骨骼的肿瘤。最近基于少量病例的研究表明，p53基因的过表达或改变在骨的高级别、临床侵袭性软骨肉瘤中经常出现。在本研究中，作者探讨了p53基因的过表达和改变与一大系列有长期随访数据的软骨肉瘤患者临床侵袭性之间的关系。
作者使用免疫组织化学、单链构象多态性和直接测序分析了158例骨软骨肉瘤中p53基因的表达和/或改变。然后将这些发现与各种临床病理参数和长期随访数据相关联。
在38.1%的骨软骨肉瘤中记录到p53基因存在过表达和/或结构改变。观察到p53基因的过表达或改变与肿瘤的组织学分级和转移的存在之间存在统计学上的显著相关性。p53无过表达或改变的患者局部无复发、无转移和总生存的概率显著高于p53过表达或改变的患者。
p53基因的过表达或改变是骨软骨肉瘤侵袭性临床行为的重要预测指标。
