两种肌动蛋白相关蛋白是染色质重塑复合物RSC和SWI/SNF的共享功能成分。

Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF.

作者信息

Cairns B R, Erdjument-Bromage H, Tempst P, Winston F, Kornberg R D

机构信息

Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.

出版信息

Mol Cell. 1998 Nov;2(5):639-51. doi: 10.1016/s1097-2765(00)80162-8.

DOI:10.1016/s1097-2765(00)80162-8

PMID:9844636

Abstract

The yeast Saccharomyces cerevisiae contains two related chromatin-remodeling complexes, RSC and SWI/SNF, which are shown to share the actin-related proteins Arp7 and Arp9. Depending on the genetic background tested, arp7 delta and arp9 delta mutants are either inviable or show greatly impaired growth and Swi-/Snf- mutant phenotypes. Unlike swi/snf mutants, viable arp7 delta or arp9 delta mutants have an Spt- phenotype, suggesting that RSC affects transcription. Temperature-sensitive mutations in ARP7 and ARP9 were isolated, and the amino acid changes support the structural relationship of Arp7 and Arp9 to actin. However, site-directed mutations predicted to impair ATP binding or hydrolysis did not detectably affect Arp7 or Arp9 function. Our results suggest that actin-related proteins perform important roles in chromatin-remodeling complexes by virtue of structural rather than enzymatic similarities to actin.

摘要

酿酒酵母含有两种相关的染色质重塑复合体，即RSC和SWI/SNF，研究表明它们共享肌动蛋白相关蛋白Arp7和Arp9。根据所测试的遗传背景，arp7δ和arp9δ突变体要么无法存活，要么生长严重受损并表现出Swi-/Snf-突变体表型。与swi/snf突变体不同，可存活的arp7δ或arp9δ突变体具有Spt-表型，这表明RSC影响转录。分离出了ARP7和ARP9中的温度敏感突变，氨基酸变化支持Arp7和Arp9与肌动蛋白的结构关系。然而，预计会损害ATP结合或水解的定点突变并未明显影响Arp7或Arp9的功能。我们的结果表明，肌动蛋白相关蛋白凭借与肌动蛋白的结构相似性而非酶活性相似性，在染色质重塑复合体中发挥重要作用。