Massidda Orietta, Anderluzzi Daniela, Friedli Laurence, Feger Georg
Department of Microbiology, Medicine Research Center,GlaxoWellcome,37100 Verona,Italy.
Geneva Biomedical Research InstitUte,GlaxoWellcome, 14 Chemin des Aulx, CH-1228 Plans-les-Ouates, Geneva,Switzerland.
Microbiology (Reading). 1998 Nov;144 ( Pt 11):3069-3078. doi: 10.1099/00221287-144-11-3069.
The genes responsible for cell wall biosynthesis and cell division (dcw genes) were identified and sequenced in Streptococcus pneumoniae. The genetic organization of the dcw cluster in Streptococcus pneumoniae differed significantly from the clusters of other bacteria reported to date. In particular, the genes corresponding to the 2 min region of the Escherichia coli chromosome were found distributed in three genetically separate regions of the Streptococcus pneumoniae chromosome. The first region contained the expected ftsA and ftsZ cell division genes at one end and pbp2b, ddl and murF at the other end. The murD, murG and divIB genes, always found located upstream of ftsA, were found in a second region separated from the first. A third region contained the yllC, yllD, pbp2x and mraY genes. The chromosomal region downstream of ftsZ was also sequenced and characterized. In Streptococcus pneumoniae this region contains four ORFs, all of unknown function, and an ORF encoding the Bacillus subtilis DivIVA homologue. The gene order and the organization of this region was found to be conserved in Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilis, raising the possibility that previously unidentified loci may also be involved in division.
在肺炎链球菌中鉴定并测序了负责细胞壁生物合成和细胞分裂的基因（dcw基因）。肺炎链球菌中dcw基因簇的遗传组织与迄今为止报道的其他细菌的基因簇有显著差异。特别是，发现与大肠杆菌染色体2分钟区域相对应的基因分布在肺炎链球菌染色体的三个遗传上独立的区域。第一个区域一端包含预期的ftsA和ftsZ细胞分裂基因，另一端包含pbp2b、ddl和murF。murD、murG和divIB基因通常位于ftsA上游，在与第一个区域分开的第二个区域中被发现。第三个区域包含yllC、yllD、pbp2x和mraY基因。ftsZ下游的染色体区域也进行了测序和表征。在肺炎链球菌中，该区域包含四个功能未知的开放阅读框（ORF）以及一个编码枯草芽孢杆菌DivIVA同源物的ORF。发现该区域的基因顺序和组织在金黄色葡萄球菌、化脓性链球菌和枯草芽孢杆菌中是保守的，这增加了以前未鉴定的基因座也可能参与细胞分裂的可能性。
