Kodama K, Fujioka T, Ito A, Nishizono A, Nasu M
Second Department of Internal Medicine, Oita Medical University, Japan.
Kansenshogaku Zasshi. 1998 Oct;72(10):1027-34. doi: 10.11150/kansenshogakuzasshi1970.72.1027.
The vacuolating cytotoxin produced by Helicobacter pylori is considered to be one virulence factor causing peptic ulceration. In this study, we examined the activity of vacuolating cytotoxin in induction of intracellular vacuolation of rabbit gastric epithelial cells (RGECs). We used culture supernatants of H. pylori as a source of vacuolating cytotoxin and quantitated cytotoxic activity by the MTT method. Intracellular vacuolation of RGECs was observed in the presence of 36 of 57 (63%) clinically isolated H. pylori strains. However, there were no differences in the incidence of H. pylori strains with positive vacuolating cytotoxin (Tox+) among patients with gastritis, gastric ulcers or duodenal ulcers. The MTT assay showed that the cytotoxic activity of H. pylori supernatants obtained from patients with gastric ulcers was significantly higher than in patients with gastritis (p < 0.01), but was not different to duodenal ulcer patient supernatants. Similar results were also observed in Tox+ isolates, however, there were no significant differences between patients with regard to the incidence of vacuolating cytotoxin-negative isolates. Although our data may not indicate a clear correlation between prevalence of vacuolating cytotoxin and clinical manifestations, they suggest that H. pylori harboring vacuolating cytotoxin may particularly induce damage to the gastric epithelium in patients with gastric ulcers.
幽门螺杆菌产生的空泡毒素被认为是导致消化性溃疡的一种毒力因子。在本研究中，我们检测了空泡毒素诱导兔胃上皮细胞（RGECs）细胞内空泡形成的活性。我们使用幽门螺杆菌的培养上清液作为空泡毒素的来源，并通过MTT法对细胞毒性活性进行定量。在57株临床分离的幽门螺杆菌菌株中，有36株（63%）存在时观察到了RGECs的细胞内空泡形成。然而，在胃炎、胃溃疡或十二指肠溃疡患者中，空泡毒素阳性（Tox+）的幽门螺杆菌菌株发生率并无差异。MTT分析表明，胃溃疡患者的幽门螺杆菌上清液的细胞毒性活性显著高于胃炎患者（p < 0.01），但与十二指肠溃疡患者的上清液无差异。在Tox+分离株中也观察到了类似结果，然而，空泡毒素阴性分离株的发生率在患者之间并无显著差异。虽然我们的数据可能并未表明空泡毒素的流行与临床表现之间存在明确的相关性，但它们表明携带空泡毒素的幽门螺杆菌可能特别会对胃溃疡患者的胃上皮造成损伤。
