Ozsan M, Tekeli A, Ozden A, Akyön Y, Ozkul A, Gerçeker D, Cetinkaya H
Department of Microbiology and Clinical Microbiology, Ankara University Faculty of Medicine, Turkey.
APMIS. 2000 Apr;108(4):293-5. doi: 10.1034/j.1600-0463.2000.d01-59.x.
In this study we examined the in vitro vacuolating cytotoxic activity of Helicobacter pylori, which is a gram-negative microaerophilic curved bacterium and a causative agent of gastritis, peptic ulcer and gastric ulcer. A vacuolating cytotoxin assay was performed to assess the vacuolating activity of 40 strains (20 gastritis, 11 gastric ulcer, and 9 duodenal ulcer), which were obtained from patients undergoing upper gastrointestinal endoscopy. The Vero cell line was used in the cytotoxic assay. Of the 40 isolates, 24 (12 gastritis, 6 gastric ulcer, 6 duodenal ulcer) were cytotoxic for the Vero cell line at 1:4 and 1:8 dilutions. Thus, vacuolating cytotoxin of H. pylori affects the Vero cell line, but it seems there is no correlation between the positivity of the strains and the risk of any particular H. pylori disease.
在本研究中，我们检测了幽门螺杆菌的体外空泡化细胞毒性活性。幽门螺杆菌是一种革兰氏阴性微需氧弯曲菌，是胃炎、消化性溃疡和胃溃疡的病原体。进行了空泡化细胞毒素检测，以评估从接受上消化道内镜检查的患者中获得的40株菌株（20株胃炎、11株胃溃疡和9株十二指肠溃疡）的空泡化活性。细胞毒性检测中使用了Vero细胞系。在40株分离株中，24株（12株胃炎、6株胃溃疡、6株十二指肠溃疡）在1:4和1:8稀释度时对Vero细胞系具有细胞毒性。因此，幽门螺杆菌的空泡化细胞毒素会影响Vero细胞系，但菌株的阳性与任何特定幽门螺杆菌疾病的风险之间似乎没有相关性。
