Duma R J, Finley R
Antimicrob Agents Chemother. 1976 Aug;10(2):370-6. doi: 10.1128/AAC.10.2.370.
Six pathogenic strains of Naegleria fowleri, two of Acanthamoeba castellanii, and three of Acanthamoeba polyphaga were tested in vitro for susceptibility to a variety of potentially useful therapeutic agents. Minimal motility inhibitory concentrations and minimal inhibitory concentrations were determined by a technique of subculturing pure clones of amoebae in plastic tissue culture chamber slides containing liquid axenic media and serially diluted drug, incubating at 30 degrees C for Acanthamoeba and at 37 degrees C for Naegleria, and observing on an inverted microscope at 6 h for inhibition of motility and at 24 and 48 h for inhibition of growth. Drug concentrations were selected on the basis of fluid levels achievable in humans. Amphotericin B, clotrimazole, and miconazole were the most effective drugs against Naegleria, whereas polymyxin B sulfate and pentamidine isethionate were somewhat effective against pathogenic Acanthamoeba. Our results suggest that amphotericin B is the most effective agent against Naegleria, but few agents are effective against Acanthamoeba.
对6株福氏耐格里阿米巴、2株卡氏棘阿米巴和3株多食棘阿米巴的致病菌株进行了体外试验，检测它们对多种潜在有效治疗药物的敏感性。通过将变形虫纯克隆接种到含有液体无菌培养基和系列稀释药物的塑料组织培养室载玻片上进行传代培养，对棘阿米巴在30℃培养，对耐格里阿米巴在37℃培养，在倒置显微镜下于6小时观察运动抑制情况，在24小时和48小时观察生长抑制情况，从而确定最小运动抑制浓度和最小抑制浓度。根据人体可达到的液体水平选择药物浓度。两性霉素B、克霉唑和咪康唑是抗耐格里阿米巴最有效的药物，而硫酸多粘菌素B和乙磺半胱氨酸喷他脒对致病性棘阿米巴有一定疗效。我们的结果表明，两性霉素B是抗耐格里阿米巴最有效的药物，但很少有药物对棘阿米巴有效。
