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农杆菌素84的化学性质及其对根癌土壤杆菌强毒株的影响。

Chemical nature of agrocin 84 and its effect on a virulent strain of Agrobacterium tumefaciens.

作者信息

McCardell B A, Pootjes C F

出版信息

Antimicrob Agents Chemother. 1976 Sep;10(3):498-502. doi: 10.1128/AAC.10.3.498.

Abstract

Agrocin 84, produced by Agrobacterium radiobacter K84, inhibited ribonucleic acid, deoxyribonucleic acid, and protein synthesis and amino acid transport in a susceptible, virulent strain of A. tumefaciens H-38-9. Cell motility was immediately stopped by action of the agrocin, 50% of the cells were killed within 15 min of contact, and the remainder were inhibited. Agrocin 84 is trypsin and pepsin resistant, but chemical analysis indicated a small peptide with a molecular weight of 2,500 containing six different amino acids, including nine molecules of glutamine or glutamic acid and seven molecules of serine.

摘要

由放射土壤杆菌K84产生的农杆菌素84,可抑制根癌土壤杆菌H - 38 - 9的敏感致病菌株中的核糖核酸、脱氧核糖核酸以及蛋白质合成和氨基酸转运。农杆菌素的作用会立即停止细胞运动,接触后15分钟内50%的细胞会死亡,其余细胞则受到抑制。农杆菌素84对胰蛋白酶和胃蛋白酶具有抗性,但化学分析表明它是一种分子量为2500的小肽,含有六种不同的氨基酸,包括九个谷氨酰胺或谷氨酸分子和七个丝氨酸分子。

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