Tutsi Yekbun, Akkus Murat, Tunik Selcuk, Nergiz Yusuf, Kaplanoglu Iskender, Nasir Yasemin
J Reprod Med. 2015 Mar-Apr;60(3-4):141-8.
Infertility is described as the inability of a couple to conceive after 1 year of unprotected intercourse. Male factor is assumed to be responsible for 50% of cases of infertile couples. It is a common clinical problem, affecting approximately 13-15% of couples worldwide. In this study we aimed to investigate the effects of hypoosmotic swelling (HOS) and anti-HOS tests on sperm membrane integrity at the ultrastructural level.
Twenty-nine infertile and 10 fertile men were included in this study. The fertile and infertile subjects were classified according to Kruger and WHO criterion. All semen samples were examined and ana-yzed based on WHO guidelines. Sperm viability was determined by using the eosin Y staining method. After HOS and anti-HOS tests wore applied, the samples were evaluated at the ultrastructural level.
Normal structural features of all regions of sperm were observed in sections of sham normospermia. Some histopathological changes wore seen in HOS and anti-HOS group sections.
The HOS procedure was found not to cause degenerative changes in the sperm ultrastructure. The anti-HOS procedure can be applied in normospermic and oligospermic groups.
不孕症被定义为一对夫妇在无保护性交1年后仍无法受孕。男性因素被认为是导致50%不育夫妇病例的原因。这是一个常见的临床问题，影响着全球约13% - 15%的夫妇。在本研究中，我们旨在从超微结构水平研究低渗肿胀（HOS）和抗HOS试验对精子膜完整性的影响。
本研究纳入了29名不育男性和10名生育能力正常的男性。根据克鲁格和世界卫生组织标准对生育能力正常和不育的受试者进行分类。所有精液样本均按照世界卫生组织指南进行检查和分析。使用伊红Y染色法测定精子活力。在进行HOS和抗HOS试验后，在超微结构水平对样本进行评估。
在假正常精液症切片中观察到精子所有区域的正常结构特征。在HOS和抗HOS组切片中可见一些组织病理学变化。
发现HOS程序不会导致精子超微结构的退行性变化。抗HOS程序可应用于正常精液症和少精子症组。
