采用基于核酸序列扩增的RNA检测法监测抗生素治疗后沙眼衣原体感染情况。
Monitoring of Chlamydia trachomatis infections after antibiotic treatment using RNA detection by nucleic acid sequence based amplification.
作者信息
Morré S A, Sillekens P T, Jacobs M V, de Blok S, Ossewaarde J M, van Aarle P, van Gemen B, Walboomers J M, Meijer C J, van den Brule A J
机构信息
Department of Pathology, University Hospital Vrije Universiteit, HV Amsterdam, The Netherlands.
出版信息
Mol Pathol. 1998 Jun;51(3):149-54. doi: 10.1136/mp.51.3.149.
AIM
To investigate the value of RNA detection by nucleic acid sequence based amplification (NASBA) for the monitoring of Chlamydia trachomatis infections after antibiotic treatment.
METHODS
Cervical smears (n = 97) and urine specimens (n = 61) from 25 C trachomatis positive female patients were analysed for the presence of C trachomatis 16S ribosomal RNA (rRNA) by NASBA and C trachomatis plasmid DNA by the polymerase chain reaction (PCR) before and up to five weeks after antibiotic treatment.
RESULTS
Chlamydia trachomatis RNA was found in all cervical smears taken before antibiotic treatment (n = 24) and in two smears taken one week after antibiotic treatment; no C trachomatis RNA was detected after two weeks or more. In contrast, C trachomatis DNA was found in all such specimens before treatment, and 21 of 25, six of 21, and five of 20 smears were found to be positive at one, two, and three weeks after treatment, respectively. After four weeks, only one of six smears was positive, and this smear had been negative in the two preceding weeks. Of the 61 urine samples investigated, C trachomatis DNA and C trachomatis RNA were found in all before treatment (n = 15), whereas one week after treatment four of 15 were C trachomatis DNA positive and C trachomatis RNA was detected in one sample only.
CONCLUSIONS
These data show that RNA detection by NASBA can be used successfully to monitor C trachomatis infections after antibiotic treatment. Furthermore, it might be possible to use urine specimens as a test of cure because neither C. trachomatis DNA or RNA could be detected two weeks or more after treatment.
目的
探讨基于核酸序列扩增技术（NASBA）检测RNA在监测沙眼衣原体感染抗生素治疗后情况中的价值。
方法
对25例沙眼衣原体阳性女性患者的宫颈涂片（n = 97）和尿液标本（n = 61），在抗生素治疗前及治疗后长达五周的时间内，采用NASBA分析沙眼衣原体16S核糖体RNA（rRNA）的存在情况，并用聚合酶链反应（PCR）分析沙眼衣原体质粒DNA。
结果
抗生素治疗前采集的所有宫颈涂片（n = 24）以及治疗后一周采集的两份涂片中均发现沙眼衣原体RNA；治疗两周或更长时间后未检测到沙眼衣原体RNA。相比之下，治疗前所有此类标本中均发现沙眼衣原体DNA，治疗后一周、两周和三周时，分别有25份涂片中的21份、21份中的6份以及20份中的5份呈阳性。四周后，6份涂片中只有1份呈阳性，且该涂片在前两周均为阴性。在检测的61份尿液样本中，治疗前所有样本（n = 15）均发现沙眼衣原体DNA和沙眼衣原体RNA，而治疗后一周，15份样本中有4份沙眼衣原体DNA呈阳性，仅在1份样本中检测到沙眼衣原体RNA。
结论
这些数据表明，NASBA检测RNA可成功用于监测抗生素治疗后的沙眼衣原体感染情况。此外，有可能将尿液标本用作治愈检测，因为治疗两周或更长时间后未检测到沙眼衣原体DNA或RNA。
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