Blumenfeld W, Hashmi N, Sagerman P
Department of Pathology, Winthrop-University Hospital, Mineola, New York 11501, USA.
Acta Cytol. 1998 Nov-Dec;42(6):1414-8. doi: 10.1159/000332177.
To determine whether three commonly used methods of obtaining and preparing cells for cytologic evaluation--touch preparation (TP), fine needle aspiration (FNA) and scrape preparation--yield essentially comparable material for evaluation or whether important differences exist between them that may influence interpretation.
FNA, TP and scrape preparations were made simultaneously on surgically excised specimens. Thirty breast, 6 lung and 12 colon lesions were studied. Each slide was assessed for qualitative and quantitative features. Cellularity was semiquantitatively scored.
Scrape preparations were the most cellular (P < .0001). Scrape and TPs often had larger tissue fragments than FNA. However, fragmentation of epithelial groups into smaller clusters and single cells was often noted on scrape preparations. FNAs tended to have cleaner backgrounds than the other two methods.
Most often, there are few differences between smears prepared by the three techniques studied. However, scrape preparations may yield smears with smaller clusters and single cells as compared to the other two methods; that is a potential source of diagnostic confusion, particularly with respect to benign breast lesions. Scrape preparations uniformly yield more cellular smears. To the extent that cellularity is an interpretive factor in assessing cytologic specimens, it is important to be aware of the increased cellularity of scrape preparations as compared to the other two techniques.
确定三种常用的获取和制备用于细胞学评估的细胞的方法——触片制备（TP）、细针穿刺抽吸（FNA）和刮片制备——所产生的用于评估的材料是否基本可比，或者它们之间是否存在可能影响判读的重要差异。
对手术切除的标本同时进行FNA、TP和刮片制备。研究了30个乳腺、6个肺和12个结肠病变。对每张玻片的定性和定量特征进行评估。对细胞数量进行半定量评分。
刮片制备的细胞数量最多（P < .0001）。刮片和触片通常比FNA有更大的组织碎片。然而，在刮片制备中经常注意到上皮细胞群破碎成较小的细胞簇和单个细胞。FNA的背景往往比其他两种方法更干净。
大多数情况下，所研究的三种技术制备的涂片之间差异不大。然而，与其他两种方法相比，刮片制备可能产生细胞簇和单个细胞较小的涂片；这是诊断混淆的一个潜在来源，尤其是对于良性乳腺病变。刮片制备始终会产生细胞数量更多的涂片。就细胞数量是评估细胞学标本的一个判读因素而言，重要的是要意识到与其他两种技术相比，刮片制备的细胞数量增加。
