Kolte Sachin S, Satarkar Rahul N
Department of Pathology, Mamata Medical College, Khammam, Andhra Pradesh, India.
J Cytol. 2010 Jul;27(3):86-90. doi: 10.4103/0970-9371.71871.
Rapid diagnosis of surgically removed specimens has created many controversies and a single completely reliable method has not yet been developed. Histopathology of a paraffin section remains the ultimate gold standard in tissue diagnosis. Frozen section is routinely used by the surgical pathology laboratories for intraoperative diagnosis. The use of either frozen section or cytological examination alone has an acceptable rate (93-97%) of correct diagnosis, with regard to interpretation of benign versus malignant.
To evaluate the utility of scrape cytology for the rapid diagnosis of surgically removed tumors and its utilisation for learning cytopathology.
75 surgically removed specimens from various organs and systems were studied. Scrapings were taken from each specimen before formalin fixation and stained by modified rapid Papanicolaou staining.
Of the 75 cases studied, 73 could be correctly differentiated into benign and malignant tumors, with an accuracy rate of 97.3%.
Intraoperative scrape cytology is useful for intraoperative diagnosis of tumor, where facilities for frozen section are not available. The skill and expertise developed by routinely practicing intraoperative cytology can be applied to the interpretation of fine needle aspirate smears. Thus, apart from its diagnostic role, intraoperative cytology can become a very useful learning tool in the field of cytopathology.
手术切除标本的快速诊断引发了诸多争议，且尚未开发出一种完全可靠的单一方法。石蜡切片的组织病理学仍是组织诊断的最终金标准。手术病理实验室常规使用冰冻切片进行术中诊断。就良性与恶性的判断而言，单独使用冰冻切片或细胞学检查的正确诊断率均可接受（93 - 97%）。
评估刮片细胞学在手术切除肿瘤快速诊断中的效用及其在学习细胞病理学方面的应用。
研究了75例来自不同器官和系统的手术切除标本。在福尔马林固定前从每个标本上取材刮片，并用改良快速巴氏染色法染色。
在所研究的75例病例中，73例能够正确区分为良性和恶性肿瘤，准确率为97.3%。
在没有冰冻切片设备的情况下，术中刮片细胞学对肿瘤的术中诊断有用。通过常规进行术中细胞学检查所培养的技能和专业知识可应用于细针穿刺涂片的判读。因此，术中细胞学除了其诊断作用外，还可成为细胞病理学领域非常有用的学习工具。
