Kilburn L, Poole K, Meyer J M, Neshat S
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
J Bacteriol. 1998 Dec;180(24):6753-6. doi: 10.1128/JB.180.24.6753-6756.1998.
Insertion of an 18-amino-acid-encoding sequence within the fpvA gene identified permissive sites at residues Y350, A402, R451, R521, and R558, consistent with these residues occurring in extramembranous loop regions of the protein. Insertions at R451, R521, and R558 did not adversely affect receptor function, although insertions at Y350 and A402 compromised ferric pyoverdine binding and uptake. The latter region likely contributes to or interacts with the ligand-binding site.
在fpvA基因内插入一个编码18个氨基酸的序列，确定了Y350、A402、R451、R521和R558残基处的允许位点，这与这些残基出现在该蛋白质的膜外环区域一致。在R451、R521和R558处的插入并未对受体功能产生不利影响，尽管在Y350和A402处的插入损害了铁载体绿脓菌素的结合和摄取。后一个区域可能对配体结合位点有贡献或与其相互作用。
