Pawlicka E, Bańkowski E, Jaworski S
Department of Biochemistry, Academy of Medicine, Bialystok, Poland.
Biol Neonate. 1999;75(2):91-6. doi: 10.1159/000014083.
Edema, proteinuria, hypertension (EPH) gestosis is accompanied by an increase of collagen content and premature replacement of hyaluronic acid by sulfated glycosaminoglycans both in the umbilical cord arteries and in Wharton's jelly. The effect of EPH gestosis on elastin content and metabolism in the umbilical cord arterial wall was the aim of this work. Studies were performed on normal umbilical cord arteries and those taken from newborns of mothers with EPH gestosis. Elastin was isolated from the arterial wall and quantified by a dye-binding method. Biosynthesis and degradation of this protein was evaluated by a pulse-chase experiment with the use of 14C-proline. It was found that EPH gestosis is associated with a significant reduction of elastin content in the umbilical cord arteries as a result of decrease in elastin biosynthesis rate and accelerated degradation of this protein. The replacement of elastin by collagen, and hyaluronate by sulfated glycosaminoglycans, may decrease the hydration of arterial wall and reduce its elasticity. Such rearrangement of extracellular matrix of the umbilical cord arteries may affect mechanical properties of these vessels and disturb fetal blood circulation.
水肿、蛋白尿、高血压（EPH）妊娠中毒症伴有脐带动脉和华通胶中胶原蛋白含量增加以及硫酸化糖胺聚糖过早取代透明质酸。本研究旨在探讨EPH妊娠中毒症对脐带动脉壁弹性蛋白含量及代谢的影响。对正常脐带动脉和EPH妊娠中毒症母亲所生新生儿的脐带动脉进行了研究。从动脉壁中分离出弹性蛋白，并采用染料结合法进行定量分析。通过使用14C-脯氨酸的脉冲追踪实验评估该蛋白的生物合成和降解。结果发现，由于弹性蛋白生物合成速率降低和该蛋白降解加速，EPH妊娠中毒症与脐带动脉中弹性蛋白含量显著降低有关。弹性蛋白被胶原蛋白取代，透明质酸盐被硫酸化糖胺聚糖取代，可能会降低动脉壁的水合作用并降低其弹性。脐带动脉细胞外基质的这种重排可能会影响这些血管的力学性能并扰乱胎儿血液循环。
