Kanauchi O, Mitsuyama K, Saiki T, Agata K, Nakamura T, Iwanaga T
Applied Bioresearch Center, Corporate Research and Development Division, Kirin Brewery Co. Ltd., Takasaki, Gunma 370-12, Japan.
Int J Mol Med. 1998 Jun;1(6):961-6. doi: 10.3892/ijmm.1.6.961.
The preventive effects of the dietary germinated barley foodstuff (GBF), which increases the contents of protein, RNA and DNA in the intestinal mucosa of rats on the mucosal damage and diarrhea were examined in a methotrexate (MTX)-induced enteritis model in rats. Sprague-Dawley rats intraperitoneally injected with MTX (10 mg/kg body weight) were used as an enteritis model. After consumption of diets containing GBF, glutamine or a glutamine-rich stuff (gluten), mucosal damage, contents of mucosal protein, RNA and DNA, myeloperoxidase (MPO) activity, bacterial translocation and DNA synthetic activity in the small intestine were assessed. GBF more effectively prevented diarrhea and mucosal damages, and increased mucosal protein, DNA and RNA contents than glutamine or gluten. The bacterial trans-location and elevation of MPO activity induced by MTX were depressed only by the consumption of GBF. GBF has a potential as therapeutic diet to decrease the adverse effects of anti-cancer chemotherapy.
在甲氨蝶呤（MTX）诱导的大鼠肠炎模型中，研究了能增加大鼠肠黏膜中蛋白质、RNA和DNA含量的发芽大麦食品（GBF）对黏膜损伤和腹泻的预防作用。将腹腔注射MTX（10毫克/千克体重）的斯普拉格-道利大鼠用作肠炎模型。在食用含GBF、谷氨酰胺或富含谷氨酰胺的物质（麸质）的饮食后，评估小肠中的黏膜损伤、黏膜蛋白质、RNA和DNA含量、髓过氧化物酶（MPO）活性、细菌移位和DNA合成活性。与谷氨酰胺或麸质相比，GBF能更有效地预防腹泻和黏膜损伤，并增加黏膜蛋白质、DNA和RNA含量。MTX诱导的细菌移位和MPO活性升高仅通过食用GBF得到抑制。GBF有潜力作为治疗性饮食来减少抗癌化疗的不良反应。
