LaRussa P, Steinberg S, Arvin A, Dwyer D, Burgess M, Menegus M, Rekrut K, Yamanishi K, Gershon A
Columbia University, New York City, New York, USA.
J Infect Dis. 1998 Nov;178 Suppl 1:S64-6. doi: 10.1086/514267.
A polymerase chain reaction (PCR) assay that identifies and differentiates wild-type (wt) and vaccine strains of varicella-zoster virus (VZV) was used to determine if VZV strains with restriction fragment length polymorphisms resembling those of the Japanese Oka vaccine strain were present in the wt pool outside of Japan. Virus samples (n = 114) from patients with chickenpox and zoster from various parts of the United States and Australia were analyzed. The assay correctly identified 113 samples as wt strain. The 1 sample identified as Oka vaccine strain came from a child with leukemia who developed a vaccine-associated rash after receiving the live attenuated varicella vaccine. At this point, there is no evidence that wt strains resembling the vaccine are circulating outside of Japan. This indicates that this PCR assay can be utilized to distinguish rashes due to vaccine and wt VZV.
一种用于鉴定和区分水痘带状疱疹病毒（VZV）野生型（wt）和疫苗株的聚合酶链反应（PCR）检测方法，被用于确定在日本境外的野生型病毒库中是否存在具有类似于日本Oka疫苗株的限制性片段长度多态性的VZV株。对来自美国和澳大利亚各地的水痘和带状疱疹患者的病毒样本（n = 114）进行了分析。该检测方法正确地将113个样本鉴定为野生型毒株。被鉴定为Oka疫苗株的1个样本来自一名白血病儿童，该儿童在接种减毒活水痘疫苗后出现了与疫苗相关的皮疹。目前，没有证据表明类似于疫苗的野生型毒株在日本境外传播。这表明该PCR检测方法可用于区分由疫苗和野生型VZV引起的皮疹。
