Janáky T, Szabó P, Kele Z, Baláspiri L, Varga C, Gálfi M, Vecsernyés M, Gáspár L, Juhász A, László F A
Department of Medical Chemistry, Albert Szent-Györgyi Medical School, Szeged, Hungary.
Rapid Commun Mass Spectrom. 1998;12(22):1765-8. doi: 10.1002/(SICI)1097-0231(19981130)12:22<1765::AID-RCM359>3.0.CO;2-U.
Our observation that dispersed cultures of neurohypophysis obtained from adult rats are capable of synthesizing and releasing oxytocin and vasopressin is unexpected, because in whole animals these hormones are known only to be stored, not to be produced in the posterior lobe of the pituitary. The hormone content of cell culture medium was elevated from 0 to 129 +/- 14 pg/mg protein for oxytocin and from 0 to 42 +/- 4 pg/mg protein for vasopressin during two weeks as determined by specific radioimmunoassay. By molecular mass and structure determination (tandem mass spectrometry) we have proved that the supernatant of the cell cultures contains not only immunologically but mass spectrometrically identified neurohypophyseal hormones.
我们观察到，从成年大鼠获得的神经垂体分散培养物能够合成和释放催产素和血管加压素，这一结果出人意料，因为在完整动物中，已知这些激素仅在垂体后叶储存，而非产生。通过特异性放射免疫测定法确定，在两周内，细胞培养基中的激素含量，催产素从0升高至129±14 pg/mg蛋白质，血管加压素从0升高至42±4 pg/mg蛋白质。通过分子量和结构测定（串联质谱法），我们已证明细胞培养物的上清液不仅含有通过免疫鉴定而且通过质谱鉴定的神经垂体激素。
