Nakasone H, Sakugawa H, Shokita H, Nakayoshi T, Kawakami Y, Kinjo F, Saito A, Shinjo M, Adaniya H, Mizushima T, Taira M
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
J Gastroenterol. 1998 Dec;33(6):850-4. doi: 10.1007/s005350050186.
The aims of this study were twofold: (1) to determine the prevalence and clinical features of hepatitis delta virus (HDV) infection among subjects positive for hepatitis B surface antigen (HBsAg) living in the Miyako Islands, Okinawa Prefecture, Japan, and (2) to clarify the relationship between HDV-RNA level and severity of HDV-related liver disease. One hundred and ninety-nine HBsAg-positive subjects (123 asymptomatic carriers [ASCs], 3 patients with acute hepatitis [AH], 50 patients with chronic hepatitis [CH], 15 patients with liver cirrhosis [LC], and 8 patients with hepatocellular carcinoma [HCC], were tested for antibody to HDV (anti-HDV) by radioimmunoassay. Anti-HDV-positive individuals were examined to determine semi-quantified HDV-RNA level by polymerase chain reaction (PCR). The overall prevalence of anti-HDV among the 199 subjects was 21.1%. The positivity rate tended to increase with age or the severity of the underlying liver disease: anti-HDV-positive rates were 10.6% (13/123) in ASCs, 32.0% (16/50) in patients with CH, 40.0% (6/15) in patients with LC, and 87.5% (7/8) in patients with HCC. None of the patients with AH were positive for anti-HDV. There was no correlation between semi-quantified serum HDV-RNA levels and the severity of chronic liver disease in patients positive for anti-HDV. The present study showed the local spread of HDV infection in the Miyako Islands, Okinawa, Japan. Although the anti-HDV positivity rate tended to increase with the severity of the underlying liver disease, the severity of HDV-related liver disease did not correlate with the semi-quantified serum HDV-RNA level.
（1）确定居住在日本冲绳县宫古岛的乙肝表面抗原（HBsAg）阳性者中丁型肝炎病毒（HDV）感染的患病率和临床特征，（2）阐明HDV-RNA水平与HDV相关肝病严重程度之间的关系。通过放射免疫分析法对199例HBsAg阳性者进行检测，其中包括123例无症状携带者（ASC）、3例急性肝炎患者（AH）、50例慢性肝炎患者（CH）、15例肝硬化患者（LC）和8例肝细胞癌患者（HCC），检测其抗HDV抗体（抗-HDV）。对抗-HDV阳性个体进行检测，通过聚合酶链反应（PCR）确定半定量HDV-RNA水平。199例受试者中抗-HDV的总体患病率为21.1%。阳性率随年龄或潜在肝病的严重程度增加而呈上升趋势：ASC中的抗-HDV阳性率为10.6%（13/123），CH患者中为32.0%（16/50），LC患者中为40.0%（6/15），HCC患者中为87.5%（7/8）。AH患者中无一例抗-HDV阳性。抗-HDV阳性患者的半定量血清HDV-RNA水平与慢性肝病的严重程度之间无相关性。本研究表明HDV感染在日本冲绳宫古岛存在局部传播。虽然抗-HDV阳性率随潜在肝病的严重程度增加而呈上升趋势，但HDV相关肝病的严重程度与半定量血清HDV-RNA水平无关。
