Zeitlin L, Olmsted S S, Moench T R, Co M S, Martinell B J, Paradkar V M, Russell D R, Queen C, Cone R A, Whaley K J
ReProtect, LLC, Baltimore, MD, USA.
Nat Biotechnol. 1998 Dec;16(13):1361-4. doi: 10.1038/4344.
The ability to produce monoclonal antibodies (Mabs) in plants offers the opportunity for the development of an inexpensive method of mucosal immunoprotection against sexually transmitted diseases. To investigate the suitability of plant-expressed Mabs for vaginal preventive applications, we compared a humanized anti-herpes simplex virus 2 (HSV-2) Mab expressed in mammalian cell culture with the same antibody expressed in soybean. We found these Mabs to be similar in their stability in human semen and cervical mucus over 24 h, their ability to diffuse in human cervical mucus, and their efficacy for prevention of vaginal HSV-2 infection in the mouse.
在植物中生产单克隆抗体（Mabs）的能力为开发一种针对性传播疾病的廉价黏膜免疫保护方法提供了机会。为了研究植物表达的单克隆抗体在阴道预防应用中的适用性，我们将在哺乳动物细胞培养物中表达的人源化抗单纯疱疹病毒2（HSV-2）单克隆抗体与在大豆中表达的相同抗体进行了比较。我们发现这些单克隆抗体在人精液和宫颈黏液中24小时内的稳定性、在人宫颈黏液中的扩散能力以及在小鼠中预防阴道HSV-2感染的效果方面相似。
