Dalton C F, Laird S M, Estdale S E, Saravelos H G, Li T C
Biomedical Research Unit, Jessop Hospital for Women, Sheffield, UK.
Hum Reprod. 1998 Nov;13(11):3197-202. doi: 10.1093/humrep/13.11.3197.
The concentrations of endometrial proteins PP14 and CA-125 were measured in uterine flushings taken on days LH+10 and LH+12 (10 and 12 days after luteinizing hormone surge) of the menstrual cycle from 15 normal, fertile women and 49 women who suffered recurrent miscarriage. The concentration of PP14 was significantly lower in the flushings from the recurrent miscarriage patients than in those from fertile controls on both day LH+10 (median: 1300, range: 3-10 300 ng/ml versus median: 13 933, range: 2174-40 404 ng/ml; P < 0.01) and LH+12 (median: 1560, range: 820-12 100 ng/ml versus median: 14 047, range 1402-62 108 ng/ml; P < 0.05). Similarly concentrations of CA-125 were significantly lower in flushings from recurrent miscarriage women compared to controls on both day LH + 10 (median: 1555, range: 47-6710 U/ml versus median: 6385.5, range 2884-27 731 U/ml, P < 0.01) and LH+12 (median: 2892, range: 956-9974 U/ml versus median: 7127.5, range: 1591-21 343 U/ml; P < 0.05). In contrast there was no significant difference in the concentration of PP14 in plasma samples taken on the same days as the flushings from recurrent miscarriage patients and fertile controls. The concentrations of PP14 in uterine flushings obtained on day LH + 10 or LH + 12 from recurrent miscarriage women during a pre-pregnancy investigative cycle were significantly lower (P < 0.05) in patients who went on to miscarry (median: 1000, range: 9-2900 ng/ml) than those who went on to have a live birth (median: 1440, range: 4-12 100 ng/ml) during a subsequent pregnancy. In contrast there was no significant difference in uterine CA-125 or plasma PP14 concentrations between these two groups of recurrent miscarriage patients. The results suggest that measurements of uterine PP14 and CA-125 may be useful in the assessment of endometrial development in recurrent miscarriage patients and suggest the importance of PP14 in preparing the endometrium for embryo implantation. In addition pre-pregnancy uterine PP14 measurements may be useful in predicting subsequent pregnancy outcome.
在月经周期中促黄体生成素激增后第10天（LH + 10）和第12天（LH + 12），从15名正常、可育女性以及49名反复流产的女性中采集子宫冲洗液，测量其中子宫内膜蛋白PP14和CA - 125的浓度。在LH + 10天，反复流产患者冲洗液中PP14的浓度显著低于可育对照组（中位数：1300，范围：3 - 10300 ng/ml；对照组中位数：13933，范围：2174 - 40404 ng/ml；P < 0.01），在LH + 12天也是如此（中位数：1560，范围：820 - 12100 ng/ml；对照组中位数：14047，范围1402 - 62108 ng/ml；P < 0.05）。同样，在LH + 10天，反复流产女性冲洗液中CA - 125的浓度与对照组相比显著降低（中位数：1555，范围：47 - 6710 U/ml；对照组中位数：6385.5，范围2884 - 27731 U/ml，P < 0.01），在LH + 12天也是这样（中位数：2892，范围：956 - 9974 U/ml；对照组中位数：7127.5，范围：1591 - 21343 U/ml；P < 0.05）。相比之下，在与反复流产患者和可育对照组冲洗液采集同一天所取血浆样本中，PP14的浓度没有显著差异。在孕前检查周期的LH + 10天或LH + 12天获得的反复流产女性子宫冲洗液中，后续发生流产的患者（中位数：1000，范围：9 - 2900 ng/ml）的PP14浓度显著低于（P < 0.05）后续活产的患者（中位数：1440，范围：4 - 12100 ng/ml）。相比之下，这两组反复流产患者的子宫CA - 125或血浆PP14浓度没有显著差异。结果表明，测量子宫PP14和CA - 125可能有助于评估反复流产患者的子宫内膜发育情况，并提示PP14在使子宫内膜为胚胎着床做准备方面的重要性。此外，孕前子宫PP14测量可能有助于预测后续妊娠结局。
