Morales M, Battenberg E, Bloom F E
The Scripps Research Institute, Department of Neuropharmacology, La Jolla, California 92037, USA.
J Comp Neurol. 1998 Dec 21;402(3):385-401.
The cellular distribution of the type 3 serotonin receptor (5HT3R) in the rat brain was established immunocytochemically by using a polyclonal antibody raised against a synthetic peptide from the deduced amino-acid sequence of the cloned 5HT3R. The 5HT3R-immunoreactive neurons were found in the forebrain, brainstem, and spinal cord, but within each region, the intensity of the immunoreactivity differed considerably. Within the forebrain, intensely immunoreactive cells were found in layers II-III of the neocortex, anterior olfactory nucleus, hippocampal formation, and amygdala. A few strongly immunoreactive neurons were consistently observed in the caudate putamen, and moderately or weakly labeled neurons were occasionally found in the nucleus accumbens. Within the brainstem, intensely labeled neurons were found in the trigeminal motor (V) and facial (VII) nuclei. Immunostained neurons were detected in the dorsal and the ventral horn of the spinal cord. These results reveal that the 5HT3R-immunoreactive neurons are broadly distributed throughout the rat brain and spinal cord, and suggest that this receptor can subserve significant participation in central nervous system neurotransmission.
通过使用针对从克隆的5HT3R推导氨基酸序列合成肽产生的多克隆抗体，免疫细胞化学法确定了大鼠脑中3型5-羟色胺受体（5HT3R）的细胞分布。在大鼠的前脑、脑干和脊髓中发现了5HT3R免疫反应性神经元，但在每个区域内，免疫反应强度差异很大。在前脑内，在新皮质的II-III层、前嗅核、海马结构和杏仁核中发现了强免疫反应性细胞。在尾状壳核中始终观察到少数强免疫反应性神经元，在伏隔核中偶尔发现中度或弱标记的神经元。在脑干内，在三叉神经运动核（V）和面神经核（VII）中发现了强标记神经元。在脊髓背角和腹角检测到免疫染色神经元。这些结果表明，5HT3R免疫反应性神经元广泛分布于大鼠脑和脊髓中，并提示该受体可能在中枢神经系统神经传递中发挥重要作用。
