Kimura H, Morita M, Yabuta Y, Kuzushima K, Kato K, Kojima S, Matsuyama T, Morishima T
Department of Pediatrics, Nagoya University School of Medicine, Nagoya, Japan.
J Clin Microbiol. 1999 Jan;37(1):132-6. doi: 10.1128/JCM.37.1.132-136.1999.
To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 10(7) copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 10(3.7) copies/microg of DNA in patients with EBV-related lymphoproliferative disorders, 10(4.1) copies/microg of DNA in patients with chronic active EBV infections, and 10(2.2) copies/microg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.
为了测量有症状的爱泼斯坦-巴尔病毒（EBV）感染患者的病毒载量，我们使用实时荧光定量聚合酶链反应（PCR）检测法来定量血液中EBV DNA的量。实时荧光定量PCR检测法能够在很宽的线性范围内检测到2至超过10⁷拷贝的EBV DNA。我们估算了有症状的EBV感染患者外周血单个核细胞（PBMNC）中的病毒载量。在EBV相关淋巴增殖性疾病患者的PBMNC中，EBV-DNA平均拷贝数为每微克DNA 10³·⁷拷贝，在慢性活动性EBV感染患者中为每微克DNA 10⁴·¹拷贝，在传染性单核细胞增多症患者中为每微克DNA 10²·²拷贝。这些数值显著高于移植后患者或无EBV相关疾病的免疫功能正常对照患者中的数值。在一名传染性单核细胞增多症患者中，随着症状缓解，病毒载量下降。有症状的EBV感染患者PBMNC中EBV DNA的拷贝数与通过原位杂交检测法确定的EBV阳性细胞数相关（r = 0.842；P < 0.0001）。这些结果表明，实时荧光定量PCR检测法对于诊断有症状的EBV感染和监测病毒载量很有用。
