细菌中甲基氰（乙腈）分解的生化途径。
The biochemical pathway for the breakdown of methyl cyanide (acetonitrile) in bacteria.
作者信息
Firmin J L, Gray D O
出版信息
Biochem J. 1976 Aug 15;158(2):223-9. doi: 10.1042/bj1580223.
[2-14C]Methyl cyanide (acetonitrile) is metabolized to citrate, succinate, fumarate, malate, glutamate, pyrrolidonecarboxylic acid and aspartate. Non-radioactive acetamide and acetate compete with 14C from methyl cyanide, and [2-14C]acetate and [2-14C]methyl cyanide are metabolized at similar rates, giving identical products. This evidence, combined with the inhibitory effect of fluoroacetate and arsenite on methyl cyanide metabolism, indicates that the pathway is: methyl cyanide leads to acetamide leads to acetate leads to tricarboxylic acid-cycle intermediates. The pathway was investigated in a species of Pseudomonas (group III; N.C.I.B. 10477), but comparison of labelling patterns suggests that it also exists in several higher plants.
[2-¹⁴C]甲基氰（乙腈）可代谢生成柠檬酸、琥珀酸、富马酸、苹果酸、谷氨酸、吡咯烷酮羧酸和天冬氨酸。非放射性的乙酰胺和乙酸盐可与甲基氰中的¹⁴C竞争，且[2-¹⁴C]乙酸盐和[2-¹⁴C]甲基氰的代谢速率相似，生成相同的产物。这一证据，再加上氟乙酸盐和亚砷酸盐对甲基氰代谢的抑制作用，表明该代谢途径为：甲基氰生成乙酰胺，乙酰胺生成乙酸盐，乙酸盐生成三羧酸循环中间体。该途径已在一种假单胞菌（第三组；英国国立生物标准与控制研究所10477）中进行了研究，但对标记模式的比较表明，它也存在于几种高等植物中。
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