Hu C C, Zhang C, Qian Q, Tatum N B
Department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio 78284-7888, USA.
J Endod. 1998 Nov;24(11):744-51. doi: 10.1016/S0099-2399(98)80166-0.
Growth factors involved in normal wound healing may promote tissue repair when applied as a direct pulp capping medication. A minimal pulp exposure was made in rat molars, a pulp capping medication was placed, and the cavity was sealed. Epidermal growth factor, basic fibroblast growth factor, insulin-like growth factor II, platelet-derived growth factor-BB, and transforming growth factor-beta 1 (TGF-beta 1)--each absorbed onto a sterile collagen membrane (BioMend; Calcitek, Carlsbad, CA)--were used separately as pulpal medicaments. Dycal, unimpregnated collagen membrane, and no medication were used as controls. Eight samples from each treatment regimen were collected 2 and 3 weeks after surgery. Pulpal soft and hard tissue responses were graded. Data were analyzed by one-way ANOVA and Tukey-Kramer tests. No significant differences were detected after 2 wk. Pulp treated with TGF-beta 1 showed significantly improved soft and hard tissue healing at week 3, compared with the procedure control. We conclude that TGF-beta 1 as a pulp-capping medication enhances reparative dentin formation in rat molars.
当作为直接盖髓药物应用时，参与正常伤口愈合的生长因子可能促进组织修复。在大鼠磨牙上造成最小程度的牙髓暴露，放置盖髓药物，然后封闭窝洞。将表皮生长因子、碱性成纤维细胞生长因子、胰岛素样生长因子II、血小板衍生生长因子-BB和转化生长因子-β1（TGF-β1）——每种都吸附到无菌胶原膜（BioMend；Calcitek，卡尔斯巴德，加利福尼亚州）上——分别用作牙髓药物。Dycal、未浸渍的胶原膜和不使用药物作为对照。在手术后2周和3周从每个治疗方案中收集8个样本。对牙髓软组织和硬组织反应进行分级。数据通过单因素方差分析和Tukey-Kramer检验进行分析。2周后未检测到显著差异。与操作对照相比，用TGF-β1处理的牙髓在第3周时软组织和硬组织愈合明显改善。我们得出结论，TGF-β1作为盖髓药物可增强大鼠磨牙修复性牙本质的形成。
