Farber B F, Wolff A G
Department of Medicine, North Shore University Hospital-Cornell University Medical College, Manhasset, New York 11030.
J Biomed Mater Res. 1993 May;27(5):599-602. doi: 10.1002/jbm.820270506.
During the pathogenesis of catheter-related urinary tract infection, bacteria attach to the catheter and migrate along a biofilm into the bladder. We studied the effect of coating silastic catheters with salicylic acid on bacterial and yeast adherence. An agar roll technique, bioluminescent assay, and radiolabeling study all produced consistent results. There was a dose related inhibition of adherence with Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumoniae, Candida albicans, and Enterococcus faecalis. Inhibition of over 90% was noted with Enterobacter and Pseudomonas. Similar results were obtained in broth and synthetic urine. A dose related reduction in adherence was seen with all gram-negative rods and yeast as measured by a radiolabeling assay. TDMAC was used to bind the salicylic acid to the catheters with > 95% inhibition of the gram-negative rods. These studies suggest the potential role of coating catheters with salicylic acid to reduce bacterial adherence and urinary tract infection.
在导尿管相关尿路感染的发病机制中，细菌附着于导尿管并沿着生物膜迁移至膀胱。我们研究了用水杨酸包被硅橡胶导尿管对细菌和酵母菌黏附的影响。琼脂滚动技术、生物发光测定法和放射性标记研究均得出了一致的结果。对铜绿假单胞菌、产气肠杆菌、肺炎克雷伯菌、白色念珠菌和粪肠球菌的黏附存在剂量相关的抑制作用。观察到肠杆菌和假单胞菌的抑制率超过90%。在肉汤和合成尿液中也获得了类似结果。通过放射性标记测定法测得，所有革兰氏阴性杆菌和酵母菌的黏附均出现剂量相关的减少。使用十六烷基三甲基氯化铵将水杨酸结合到导尿管上，对革兰氏阴性杆菌的抑制率>95%。这些研究表明用水杨酸包被导尿管在减少细菌黏附和尿路感染方面的潜在作用。
