Innes J F, Sharif M, Barr A R
Department of Clinical Veterinary Science, University of Bristol, United Kingdom.
Am J Vet Res. 1998 Dec;59(12):1530-6.
To investigate the hypothesis that concentrations of the keratan sulfate epitope, 1/20/5D4 (5D4) and total sulfated glycosaminoglycans (S-GAG) in synovial fluids, and of 5D4 in serum of dogs with naturally acquired osteoarthritis of the genual joint (stifle), secondary to cranial cruciate ligament deficiency, are associated with other disease parameters of osteoarthritis.
58 dogs with stifle osteoarthritis secondary to naturally acquired cranial cruciate ligament (CCL) deficiency.
All dogs were examined clinically, radiographically and, in some instances, scintigraphically. Serum and synovial fluid from both stifles were assayed for 5D4 concentration, using an inhibition ELISA, and for S-GAG, using a direct dye-binding assay.
Serum 5D4 concentration was not significantly associated with other disease features. Total S-GAG values were high, and 5D4 values were low, in synovial fluid from clinically active, compared with contralateral joints. The S-GAG concentration in synovial fluid from clinically active joints was negatively correlated with radiographic severity score (rs = -0.389, P = 0.004, Spearman's rank correlation). Other associations between marker concentrations and disease parameters could not be detected.
Serum 5D4 concentration is not a useful marker of stifle osteoarthritis in dogs; however, synovial fluid S-GAG and 5D4 values may hold more promise as disease measures.
探讨以下假设，即患有自然获得性膝关节（ stifle ）骨关节炎且继发于颅交叉韧带缺乏症的犬的滑液中硫酸角质素表位 1/20/5D4 （ 5D4 ）和总硫酸化糖胺聚糖（ S-GAG ）的浓度，以及血清中 5D4 的浓度与骨关节炎的其他疾病参数相关。
58 只患有自然获得性颅交叉韧带（ CCL ）缺乏症继发的膝关节骨关节炎的犬。
对所有犬进行临床、放射学检查，在某些情况下还进行闪烁扫描检查。使用抑制 ELISA 法检测双侧膝关节的血清和滑液中 5D4 的浓度，使用直接染料结合法检测 S-GAG 的浓度。
血清 5D4 浓度与其他疾病特征无显著相关性。与对侧关节相比，临床活动期关节滑液中的总 S-GAG 值较高，而 5D4 值较低。临床活动期关节滑液中的 S-GAG 浓度与放射学严重程度评分呈负相关（ rs = -0.389 ， P = 0.004 ， Spearman 等级相关性）。未检测到标志物浓度与疾病参数之间的其他关联。
血清 5D4 浓度不是犬膝关节骨关节炎的有用标志物；然而，滑液 S-GAG 和 5D4 值作为疾病指标可能更有前景。
