人免疫球蛋白G的渗透测定研究。
Osmometric studies of human immunoglobulin G.
作者信息
Dolecek V, Regent A, Lapanje S
出版信息
J Immunol Methods. 1978;22(3-4):319-25. doi: 10.1016/0022-1759(78)90039-x.
PMID:98588
Abstract
Osmometric studies of solutions of IgG and its H and L chains were performed in various solvents. It has been found that in aqueous solutions and in guanidinium chloride solutions of low and moderate concentrations IgG molecules are associated which prevents molecular weight determination. On the other hand, in 6 M guanidinium chloride the molecular weight of IgG was determined to be 145,000. Addition of sodium dodecyl sulfate to aqueous solutions of IgG does not prevent association.
摘要
对免疫球蛋白G（IgG）及其重链和轻链的溶液进行了在各种溶剂中的渗透压测定研究。已发现，在水溶液以及低浓度和中等浓度的氯化胍溶液中，IgG分子会缔合，这妨碍了分子量的测定。另一方面，在6M氯化胍中，IgG的分子量测定为145,000。向IgG水溶液中添加十二烷基硫酸钠并不能阻止缔合。
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