Eigenbrodt E, Kallinowski F, Ott M, Mazurek S, Vaupel P
Institute of Biochemistry and Endocrinology, Veterinary Faculty, University of Giessen, Germany.
Anticancer Res. 1998 Sep-Oct;18(5A):3267-74.
The interaction between glycolysis, glutaminolysis and tumor growth in WAG/Fra rnu/rnu rats has been investigated. Small tumors are characterized by a low conversion of glucose to lactate whereas the conversion of glutamine to lactate is high. In medium sized tumors the flow of glucose to lactate as well as oxygen utilization are increased whereas glutamine and serine consumption are reduced. At this stage the tumor cells start with glutamate and alanine production. Large tumors are characterized by a low oxygen and glucose supply but a high glucose and oxygen utilization rate. The conversion of glucose to glycine, alanine, glutamate, glutamine, and proline reaches high values and the amino acids are released. Pyruvate kinase increases with tumor weight and is positively correlated with an increase in glucose and oxygen utilization. The shift from glutamate consumption to glutamate production is correlated with an increase in glutamate dehydrogenase and glutamate oxaloacetate transaminase activity.
已对WAG/Fra rnu/rnu大鼠中糖酵解、谷氨酰胺分解与肿瘤生长之间的相互作用进行了研究。小肿瘤的特征是葡萄糖向乳酸的转化率较低，而谷氨酰胺向乳酸的转化率较高。在中等大小的肿瘤中，葡萄糖向乳酸的流量以及氧利用率增加，而谷氨酰胺和丝氨酸的消耗减少。在此阶段，肿瘤细胞开始产生谷氨酸和丙氨酸。大肿瘤的特征是氧和葡萄糖供应较低，但葡萄糖和氧利用率较高。葡萄糖向甘氨酸、丙氨酸、谷氨酸、谷氨酰胺和脯氨酸的转化率达到很高的值，并且氨基酸被释放。丙酮酸激酶随肿瘤重量增加而增加，并且与葡萄糖和氧利用率的增加呈正相关。从谷氨酸消耗向谷氨酸产生的转变与谷氨酸脱氢酶和谷氨酸草酰乙酸转氨酶活性的增加相关。
