Poovorawan Y, Theamboonlers A, Jantaradsamee P, Kaew-in N, Hirsch P, Tangkitvanich P
Dept. of Pediatrics, Faculty of Medicine, Chulalongkorn University and Hospital, Bangkok, Thailand.
Infection. 1998 Nov-Dec;26(6):355-8. doi: 10.1007/BF02770835.
The novel hepatitis TT virus first described by a Japanese group has been reported to be parenterally transmitted and furthermore, to have been detected in patients with hepatitis of unknown etiology. Hence, in the present study its prevalence was investigated within groups at high risk for contracting blood-borne viruses, such as individuals with chronic liver disease, intravenous drug users and recipients of blood and blood products, as compared to voluntary blood donors and pregnant women. To that end, DNA was extracted from sera obtained from the respective patients and subjected to PCR using semi-nested primers. The frequency of TTV DNA detected within high risk groups, such as nine out of 50 patients with chronic non-A-to-G liver disease (18%), nine out of 98 hepatocellular carcinoma cases (9.2%), 17 out of 52 intravenous drug users (32.7%), 15 out of 80 thalassemia patients with multiple blood transfusions (18.8%) and three out of 31 prostitutes (9.7%) exceeded that among voluntary blood donors and pregnant women, which amounted to 14 out of 200 (7%) and seven out of 103 (6.8%), respectively. Additional molecular research should be performed in order to determine its short-, as well as long-term clinical significance.
由一个日本研究团队首次描述的新型丁型肝炎病毒据报道可经肠道外传播，此外，在病因不明的肝炎患者中也检测到了该病毒。因此，在本研究中，与自愿献血者和孕妇相比，对感染血源病毒风险较高的人群进行了该病毒感染率的调查，这些人群包括慢性肝病患者、静脉吸毒者以及血液和血液制品接受者。为此，从各个患者的血清中提取DNA，并使用半巢式引物进行聚合酶链反应（PCR）。在高风险组中检测到丁型肝炎病毒DNA的频率超过了自愿献血者和孕妇中的频率，例如，50例慢性非甲至庚型肝病患者中有9例（18%）、98例肝细胞癌患者中有9例（9.2%）、52例静脉吸毒者中有17例（32.7%）、80例多次输血的地中海贫血患者中有15例（18.8%）、31名妓女中有3例（9.7%），而自愿献血者和孕妇中的检测率分别为200例中有14例（7%）和103例中有7例（6.8%）。为了确定其短期和长期的临床意义，应进行进一步的分子研究。
