Heywood S M, Kennedy D S
Biochemistry. 1976 Jul 27;15(15):3314-9. doi: 10.1021/bi00660a023.
Myosin messenger ribonucleoprotein-translational control ribonucleic acid (mRNP-tcNA) from myosin mRNPs found in embryonic chick muscle has been further purified by Dowex chromatography and, from a number of controls, it is suggested that this small RNA is not an artifact produced through the degradation of RNA during its isolation. This highly purified myosin mRNP-tcRNA is shown to have a molecular weight of 10 000 on formamide-acrylamide gels, and reacts stoichometrically (on a 1:1 mole ratio) with myosin mRNA. The stoichiometric interaction between myosin mRNA and myosin mRNP-tcRNP is demonstrated by ists ability to increase the nuclease resistance of the messenger, as well as inhibit its translation in a cell-free amino acid incorporating system.
从胚胎期鸡肌肉中发现的肌球蛋白信使核糖核蛋白-翻译控制核糖核酸（mRNP-tcNA），通过Dowex柱色谱法进一步纯化。从一系列对照实验来看，表明这种小RNA并非在分离过程中因RNA降解而产生的人为产物。这种高度纯化的肌球蛋白mRNP-tcRNA在甲酰胺-丙烯酰胺凝胶上显示分子量为10000，并且与肌球蛋白mRNA以化学计量比（1:1摩尔比）发生反应。肌球蛋白mRNA与肌球蛋白mRNP-tcRNP之间的化学计量相互作用通过以下方式得以证明：它能够提高信使的核酸酶抗性，以及在无细胞氨基酸掺入系统中抑制其翻译。
