Callan M F, Annels N, Steven N, Tan L, Wilson J, McMichael A J, Rickinson A B
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, GB.
Eur J Immunol. 1998 Dec;28(12):4382-90. doi: 10.1002/(SICI)1521-4141(199812)28:12<4382::AID-IMMU4382>3.0.CO;2-Z.
Memory T cell responses are frequently highly restricted in terms of receptor usage. How and when such clonotypic dominance is established remains poorly understood. Here we have investigated the evolution of the T cell responses to an epitope from Epstein-Barr virus (EBV), (FLRGRAYGL), by analyzing TCR use of clones specific for this epitope, derived from peripheral blood mononuclear cells taken from individuals early during primary EBV infection and up to 3 years later. We show that, in a given individual, particular T cell clonotypes are selected early during the primary response to this epitope and that the same clonotypes dominate the late memory response. In one individual direct analysis of HLA-B8-restricted FLRGRAYGL-specific T cells, isolated from peripheral blood lymphocytes taken during primary EBV infection using a tetrameric MHC-peptide complex, confirmed the early selection of the dominant clonotypes.
记忆性T细胞反应在受体使用方面常常受到高度限制。这种克隆型优势是如何以及何时建立的，目前仍知之甚少。在这里，我们通过分析来自原发性EB病毒（EBV）感染早期及之后3年内个体外周血单个核细胞中针对该表位（FLRGRAYGL）的克隆的TCR使用情况，研究了T细胞对EBV表位的反应演变。我们发现，在特定个体中，特定的T细胞克隆型在对该表位的初次反应早期就被选择出来，并且相同的克隆型在晚期记忆反应中占主导地位。在一名个体中，使用四聚体MHC - 肽复合物从原发性EBV感染期间采集的外周血淋巴细胞中分离出的HLA - B8限制性FLRGRAYGL特异性T细胞的直接分析，证实了优势克隆型的早期选择。
